Notch and the DSL Notch ligands Delta and Serrate/Jagged are glycoproteins with a single transmembrane domain. The extracellular domain (ECD) of both Notch receptors and Notch ligands contains numerous epidermal growth factor (EGF)-like repeats which are post-translationally modified by a variety of glycans. Inactivation of a subset of genes that encode glycosyltransferases which initiate and e...

Two glycosyltransferases that transfer sugars to EGF domains, OFUT1 and Fringe, regulate Notch signaling. However, sites of O-fucosylation on Notch that influence Notch activation have not been previously identified. Moreover, the influences of OFUT1 and Fringe on Notch activation can be positive or negative, depending on their levels of expression and on whether Delta or Serrate is signaling t...

Mammalian Notch receptors contain 29-36 epidermal growth factor (EGF)-like repeats that may be modified by protein O-fucosyltransferase 1 (Pofut1), an essential component of the canonical Notch signaling pathway. The Drosophila orthologue Ofut1 is proposed to function as both a chaperone required for stable cell surface expression of Notch and a protein O-fucosyltransferase. Here we investigate...

In this paper, Microstrip patch antenna fed through an aperture coupled 50Ω microstrip line is designed for dualband and tripleband applications by cutting U-slots in the patch. When a U-slot is cut in the patch, a notch is introduced within the matching band, results in dualband antenna. If another U-slot is cut in the same patch, antenna becomes a tripleband antenna. For better coupling apert...

The trans-admittance-mode (TAM) might act as a transforming bridge for voltage-mode to current-mode conversion. In this study a new low voltage operated electronically tunable TAM biquad filter structure realizing all the seven standard filtering functions namely; low-pass (LP), band-pass (BP), high-pass (HP), regular-notch (RN), low-pass-notch (LPN), high-pass-notch (HPN) and all-pass (AP) fro...

In this paper, accumulation of plastic deformation of AISI 1045 steel plates with circular cutouts under cyclic axial loading is studied. Loading was applied under force-control conditions. Experimental tests were performed using a Zwick/Roell servo hydraulic machine. Under force-control loading with nonzero mean force, plastic strain was accumulated in continuous cycles called ratcheting. Nume...

Notch in Invertebrate Development. Notch proteins are highly conserved transmembrane receptors that are involved in cell fate regulation in invertebrates (1). While Notch receptors are initially synthesized as single polypep-tide chains proteolytic processing results in the formation of a heterodimeric receptor in which the extracellular domain is noncovalently attached to the transmembrane and...

Multicellular development requires the correct spatial and temporal regulation of cell division and differentiation. These processes are frequently coordinated by the activities of various signaling pathways such as Notch signaling. From a screen for modifiers of Notch signaling in Drosophila we have identified the RNA helicase Belle, a recently described component of the RNA interference pathw...

BACKGROUND Ligand-induced proteolytic cleavage and internalization of the plasma membrane receptor Notch leads to its activation. Ligand-independent, steady-state internalization of Notch, however, does not lead to activation. The mechanism by which downstream effectors discriminate between these bipartite modes of Notch internalization is not understood. Nedd4 is a HECT domain-containing E3 ub...

A hexagonal ferrite-based millimeter wave notch filter was demonstrated. The filter consists of an M-type BaFe12O19 BaM slab sitting on top of a stripline. The band-stop filtering response originates from the ferromagnetic resonance absorption in the BaM slab. The BaM slab has an in-plane uniaxial anisotropy field of 17 kOe. This anisotropy field facilitates the operation of the filter beyond 5...