Enterococci are among the common organisms associated with hospital-acquired infections. We examined in vitro activities of different antibiotics to 103 enterococcal isolates. Minimal inhibitory concentrations (MICs) of penicillin G, ampicillin, gentamicin, ciprofloxacin, ofloxacin, levofloxacin, grepafloxacin, trovafloxacin and gemifloxacin were determined by broth microdilution testing method...

Multidrug-resistant (MDR) and pathogenic E. faecium is a crisis in healthcare settings. This survey aimed at antibiotic susceptibility profiling virulence determinants of faecium. study pooled 100 fecal isolates identified by phenotypic molecular tests. Antibiotic ampicillin MIC were determined according to clinical laboratory standards institute (CLSI) 2017. Moreover, biofilm formation was ass...

From 125 separate cloacal cultures from three turkey flocks fed virginiamycin, 104 Enterococcus faecium and 186 Enterococcus faecalis isolates were obtained. As the turkeys aged, there was a higher percentage of quinupristin-dalfopristin-resistant E. faecium isolates, with isolates from the oldest flock being 100% resistant. There were no vancomycin-resistant enterococci. Results of pulsed-fiel...

Twenty-eight quinupristin-dalfopristin-resistant isolates of Enterococcus faecium from hospital patients and nonhuman sources in European countries were studied. High-level resistance (MICs, >/=32 microg/ml) was associated with the presence of vat(E) (satG) (14 isolates ¿50%) or vat(D) (satA) (6 isolates ¿21%). These genes were not detected in eight (29%) isolates with lower levels of quinupris...

We determined the species distribution and prevalence of ampicillin resistance, high-level gentamicin resistance (HLGR) and vancomycin resistance among clinical enterococcal isolates from five Nordic laboratories (Bergen, Tromsø, Uppsala, Aarhus and Reykjavik). Isolates represented three different groups: (i) all blood culture isolates from 1999; (ii) consecutive in-patient isolates (maximum 40...

OBJECTIVES Vancomycin-resistant enterococci (VRE), particularly vancomycin-resistant Enterococcus faecium (VREfm), have emerged among the leading pathogens causing hospital-acquired infections worldwide. We aimed to examine whether there were newly introduced clones contributing to this increase and to assess the risk factors for mortality in patients with VREfm bacteraemia. METHODS Between 2...

OBJECTIVES We evaluated the genetic and molecular basis of high-level resistance to gentamicin and amikacin in 91 clinical isolates of Enterococcus faecalis and Enterococcus faecium in a university hospital in southern Italy from 1987 to 2003. METHODS Antibiotic susceptibility was evaluated by disc diffusion and microdilution methods. Genotyping was performed by PFGE and dendrogram analysis. ...

background in recent decades, bacterial antibiotic resistance (especially in enterococci) has become a significant problem for human and veterinary medicine. one of the most important antibiotic resistances in enterococci, vancomycin resistance, is encoded by van gene family. objectives the aim of this study was to investigate antibiotic resistance to vancomycin in enterococci and the genes res...

OBJECTIVES Ireland has the highest rate of vancomycin-resistant Enterococcus faecium (VREfm) isolated from blood of nosocomial patients in Europe, which rose from 33% (110/330) in 2007 to 45% (178/392) in 2012. No other European country had a VREfm rate from blood cultures of >25%. Our aim was to elucidate the reasons for this significantly higher rate in Ireland. METHODS The epidemiology and...