BACKGROUND The intraperitoneal injection is a common method for establishing the experimental animal model infected with parasites. The aim of this study was to investigate if the intraperitoneal injection was another route of Trichinella spiralis infection. METHODS From June to July 2015, twenty BALB/c mice were intraperitoneally injected with 300 T. spiralis muscle larvae in Department of P...

The aim of the study was to isolate genes coding for stage-specific antigens of T. spiralis. Such antigens may then be associated with local and systemic immune responses against adult T. spiralis. Recombinant clones were obtained with an adult stage specific probe from a cDNA library of three-day old adult T. spiralis. Several cDNA clones encoding the same peptide were identified and their sta...

TsPmy is a paramyosin expressed by parasitic Trichinella spiralis and confers a protective immunity when its recombinant protein or DNA was used as an immunogen. To improve its immunogenicity and vaccine efficacy, we conducted a heterologous prime-boost strategy by orally delivering one dose of TsPmy DNA carried by attenuated Salmonella typhimurium (SL7207), followed by two doses of recombinant...

The transfer of matter and energy in planktonic food webs is determined by the encounter rate between predators and prey and subsequent attack and capture success. We tested if differences in swimming behavior and escape ability of 2 planktonic ciliates could explain differences in their susceptibility to predation from the calanoid copepod Acartia clausi. We chose 2 similarly sized ciliates, w...

Enteric nematode infections are characterized by both peripheral and tissue eosinophilia. The cytokine interleukin (IL)-5 is considered a critical factor in the proliferation and recruitment of eosinophils, however, studies suggest it plays little role in host defence, at least during primary Trichinella spiralis infections. Less is known concerning its role in host defence or in the inflammato...

Trichinella spiralis has been reported to induce angiogenesis for nutrient supply and waste disposal by the induction of the angiogenic molecule vascular endothelial cell growth factor (VEGF) during nurse cell formation. However, the action mechanism to induce VEGF in nurse cells by T. spiralis is not known. Hypoxia in nurse cells was suggested as a possible mechanism; however, the presence of ...

The molluscan fauna inhabiting the algal turfs of mesolittoral rocky outcrops, dominated by coralline alga Ellisolandia elongata, has been quantitatively analysed in a Marine Protected Area Málaga, southern Spain (W Mediterranean). A total 2520 mollusc specimens belonging to 46 species were identified. This taxocenosis was compared that previously studied from infralittoral level, where Halopte...

LARSH, JOHN E., JR., OTTOLENGHI, ATHOS, AND WEATHERLY, NORMAN F. 1975. Trichinella spiralis: Phospholipase in sensitized mice after challenge. Experimental Parasitology 37, 233-238. Mice given three sensitizing infections with Trichinella spiralis and then challenged with 400 larvae showed greatly elevated phospholipase B levels in the small intestine from one through 20 days after challenge; b...

The present study was undertaken to identify potentially immunoreactive proteins of the muscle larvae (ML) and adult stage (Ad) of the nematode Trichinella spiralis Owen, 1835. To identify immunoreactive proteins that are specifically recognised by anti-Trichinella antibodies, ML and Ad crude extracts and their excretory-secretory (E-S) products were subjected to sodium dodecyl sulfate polyacry...

In this study, the effects of orally administered viable Lactobacillus casei Shirota strain YIT9029 on the immunity parameters of Wistar and Brown Norway rats were examined. For this purpose, we used the Trichinella spiralis host resistance model. Two weeks before and during T. spiralis infection, rats were fed 10(9) viable L. casei bacteria 5 days per week. The T. spiralis-specific delayed-typ...