Mouse FM3A mammary adenocarcinoma cells exposed to the specific thymidylate synthetase (TS) inhibitor 10-propargyl-5,8-dideazafolate (PDF) responded by overproducing TS up to 200-fold. In the absence of inhibitor, the elevation of TS levels decayed with a half-life of about 4 weeks. Southern blot analysis of restricted DNA from the PDF-resistant cells using a TS-specific probe showed that the T...

This study aims to design some dual-target anticancer candidates, capable to act as an alkylating agent as well as a thymidylate synthase (TS) inhibitor. The designed scaffold is a combination of nucleobase, amino acid and aziridine structures. The candidates are docked into TS and three DNA double strand structures and evaluated based on their binding interaction energies and ligand efficienci...

Examined children's ratings of attitudes and behavioral intentions toward a boy presented, on videotape, with or without symptoms of Tourette syndrome (TS). Effects of information about TS on these ratings were investigated as well as the influence of gender and grade. Children in Grades 3 and 5 were randomly assigned to one of three conditions: No TS, TS, or TS/information. On the attitude mea...

Temperature sensitivity of frog taste cells was studied. The taste cell designated Type thermosensitive (TS) I cell was depolarized by warm stimulus at 30 degrees C and hyperpolarized by cold stimulus at 10 degrees C. The taste cell designated Type TS II cell was depolarized by the cold stimulus and hyperpolarized by the warm stimulus. Menthol solution at 20 degrees C, which selectively activat...

This report describes a Ts costimulator assay and its use to analyze cofactors required for the expression of suppressor T cell function. Activation of primed MLR-Ts (alloantigen-activated suppressor T cells suppressive of mixed leukocyte reaction) to suppressor T cell factor (TsF) production typically fails in the presence of glutaraldehyde-fixed rather than irradiated allogeneic stimulator ce...

Tumor cell resistance to fluoropyrimidines and other inhibitors of thymidylate synthase (TS) is a serious problem often associated with increased intracellular TS. Clinically, another problem that arises from the use of TS inhibitors is toxicity, which develops, in part, because normal cells may be adversely affected by doses of inhibitor that do not impact tumor cells. To circumvent this probl...