PURPOSE To determine the effect of zinc on retinal cells at concentrations at which it is known to cause oxidative stress. Furthermore, the effects of metipranolol, known to prevent retinal damage, and of other antiglaucoma drugs were determined on zinc-injured retinal cells. METHODS Lipid peroxidation assays were conducted on rat brain and bovine retina-retinal pigment epithelial (RPE) membr...

Mung bean (Vigna radiata) sprout is commonly consumed as a vegetable, while the coat of the germinated mung bean is a waste. In this paper, an ultrasound-assisted extraction method has been developed to extract natural antioxidants from the seed coat of mung bean. Several experimental parameters-which included ethanol concentration, solvent/material ratio, ultrasound extraction time, temperatur...

A new formulation of bologna-type sausage enriched in ω-3 polyunsaturated fatty acids (PUFA) (8.75% linseed oil) was developed, using a lyophilized aqueous-ethanolic extract of Melissa officinalis. A comparison with the effectiveness of butylhydroxy anisole (BHA) synthetic antioxidant to decrease the oxidation of PUFAs was performed. The formulation increased the ω-3 PUFAs content, especially α...

Ischemia, and subsequent acidosis, induces neuronal death following brain injury. Oxidative stress is believed to be a key component of this neuronal degeneration. Acute chemical ischemia (azide in the absence of external glucose) and acidosis (external media buffered to pH 6.0) produce increases in intracellular calcium concentration ([Ca2+]i) and inward membrane currents in cultured rat corti...

The study shows the changes of total polyphenol compounds and antioxidant activities of Polygonum multiflorum Thunb root extract during the preservation time by freezing method. In addition, the treatment of initially raw materials by drying process also affects strongly total polyphenol compounds and antioxidant activity. Total phenolic compounds were determined by the Folin Ciocalteu method a...

Cervical spinal cord injury (SCI) can dramatically impair diaphragm muscle function and often necessitates mechanical ventilation (MV) to maintain adequate pulmonary gas exchange. MV is a life-saving intervention. However, prolonged MV results in atrophy and impaired function of the diaphragm. Since cervical SCI can also trigger diaphragm atrophy, it may create preconditions that exacerbate ven...

Treatment of free radical pathologies by antioxidants has been substantiated by studies in animal models of diseases. However, so far the therapy of oxidative stress-related diseases has not found satisfactory application in clinical practice. This may be due to an insufficient efficacy of the antioxidants available, their unsuitable pharmacokinetics, lack of selectivity, presence of adverse si...

The free radical scavenging activity of Trolox was studied for aqueous and lipid environments using the Density Functional Theory. Several reaction mechanisms and free radicals of different chemical nature have been included in this study, as well as the influence of the pH. Trolox was found to be a powerful ˙OH and alkoxy scavenger, regardless of the conditions under which the reaction takes p...

Peptides with bioactive properties can be released from the precursor proteins during food processing or digestion, and act as antioxidative compounds. Although, it has been proven that hydrolysate exerts higher antioxidant activity than purified peptides. The process of enzyme immobilization proved to be more practical and advantageous for catalysis than using free enzyme. The aim of this stud...

OBJECTIVE We investigated whether melatonin is present in walnuts (Juglans regia L.) and, if so, tested whether eating walnuts influences melatonin levels and the total antioxidant status of the blood. METHODS Melatonin was extracted from walnuts and quantified by high-performance liquid chromatography. After feeding walnuts to rats, serum melatonin concentrations were measured using a radioi...