Bed bugs Cimex lectularius L. were exposed to conidia (spores) of the entomopathogenic fungus Metarhizium anisopliae by feeding, aerosol spray, or contact with a treated surface. Feeding experiments demonstrated that bed bugs were innately susceptible to this fungus. However, only at 98% humidity were mortality rates high, regardless of whether bed bugs were sprayed with a fungal solution or co...

We present two cases of keratitis due to Metarhizium anisopliae in geographically separated areas of the United States. The isolates were microscopically similar but morphologically different and were identified by ribosomal DNA sequencing. Both isolates had low minimum inhibitory concentration (MIC) values to caspofungin and micafungin, but high MIC values to amphotericin B. The morphologic an...

Mortality of rice weevil, Sitophilus oryzae (L.) caused by Metarhizium anisopliae (Metschnikoff) Sorokin alone and in combination with a neonicotinoid insecticide, acetamiprid was evaluated in laboratory bioassays. Spray application of M. anisopliae alone at a spore concentration of 6 x 10 conidia/ml required 6 days to cause 10 percent mortality whereas 4 x 10 conidia/ml required 8 days to caus...

Entomopathogenic fungi are naturally occurring pathogens of some insects that produce enzymes capable of penetrating into an insect’s cuticle. Chitinases are thought to be responsible for entomopathogenic fungi’s ability to degrade cuticle. Primers were designed for the chi3 gene which encodes a chitinase CHIT30, from the mRNA transcript present in Metarhizium anisopliae. These primers were una...

Cecropins are the most potent induced peptides to resist invading microorganisms. In the present study, two full length cDNA encoding cecropin2 (Px-cec2) and cecropin3 (Px-cec3) were obtained from P. xylostella by integrated analysis of genome and transcriptome data. qRT-PCR analysis revealed the high levels of transcripts of Px-cecs (Px-cec1, Px-cec2 and Px-cec3) in epidermis, fat body and hem...

The susceptibility of Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) to selected strains of the entomopathogenic fungi Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin was evaluated through bioassays with direct immersion or foliar exposureunder laboratoryconditions.Results showed thatA.planipennisadultswere susceptible toB. bassiana andM. anisop...

The focus of this study was on quantifying fitness attributes, such as spore density and viability, and determining the virulence level against aphid (Aphis gossypii) nymphs of isolates from the fungal species Beauveria bassiana and Metarhizium anisopliae. The fungal isolates were obtained from several insects, including Plutella xylostella, Hypothenemus hampei, Bronstispa longissima, A. gossyp...

The use of biological control agents such as entomopathogenic fungi is an alternative for the kale(Brassica oleracea L.) defoliating caterpillars. objective was to investigate efficacy entomopathogenicfungi Amazon region in caterpillars kale and their impacts on physiologicaland agronomic responses crop. Field greenhouse experiments were conducted randomized blockdesign completely design, respe...

In the present study, pathogenicity of four native strains of Entomopathogenic fungus; Metarhizium anisopliae, was studied against adult stage of Ixodes ricinus. For this purpose a total number of 180 adult ticks were examined in triplicate. Thirty ticks for each strain and negative and positive controls were immersed in 2.4×107 fungal conidia/ml in vitro. Samples were incubated in separate Pet...

b a ckground: the protective effect of two isolates of an entomopathogenic fungus, metarhizium anisopliae (demi 002 and iran 437c) on the adult stage of varroa destructor was evaluated in comparison with fluvalinate strips in the field. m e t hods: a total of 12 honey bee colonies were provided from an apiculture farm. the selected hives were divided into 4 groups (3 hives per group). the first...