(11S)-2 -Bromo-3-oxoeudesmano-12,6 -lactone, designated santonin-related compound 2 (SRC2), only weakly affected I B degradation after tumor necrosis factor(TNF) stimulation, but strongly blocked the nuclear translocation of nuclear factor B (NFB) subunit p65. Replacement of Cys-38 of p65 with serine abolished the inhibitory effect of SRC2 on this TNFinduced nuclear translocation. These results...

Osteosarcoma (OS) is an aggressive tumor with a rare incidence. Extended surgical resections are the prevalent treatment for OS, which may cause critical-size bone defects. These defects lead to dysfunction, weakening post-surgical quality of patients’ life. Hence, ideal therapeutic agent OS should simultaneously possess anti-cancer and repair capacities. Curcumin (CUR) has been reported in the...

We describe an in vitro system with all components derived from the yeast Saccharomyces cerevisiae that can translocate a yeast secretory protein across microsomal membranes. In vitro transcribed prepro-alpha-factor mRNA served to program a membrane-depleted yeast translation system. Translocation and core glycosylation of prepro-alpha-factor were observed when yeast microsomal membranes were a...

OBJECTIVE Recent studies have demonstrated the therapeutic effectiveness and pharmacologic mechanisms of hyperbaric oxygen therapy (HBOT) in the treatment of a systemic shock state. To elucidate the in vivo role of HBOT during sepsis, we evaluated the effects of HBOT on intestinal mucosal injury and bacterial translocation after lipopolysaccharide challenge. DESIGN Experimental study. SETTI...

Protein synthesis is the result of a series of coupled events, many of which can be measured. In this report, the determination of the synthesis of peptides of chain length three and greater was used as a measure of translocation, the movement of the ribosome along the polyribonucleotide template. Translocation was observed both in the presence and absence of one added soluble transfer factor w...

PURPOSE To investigate the roles of activation of macrophages isolated from C3H/HeN and C3H/HeJ mice and stimulated by lipopolysaccharide (LPS), and toll-like receptor 4-mediated signal transduction in the development of acute anterior uveitis. METHODS Establish animal models with acute anterior uveitis by intraperitoneal injection of vibrio cholera endotoxin into C3H/HeN mice (wild type) and...

Botulinum neurotoxins and tetanus are the strongest known toxins that cause neuroparalytic syndromes in botulism tetanus. This review aimed to systematize scientific data on structures mechanism of actions botulinum neurotoxins. proteins containing functional domains responsible for receptor binding, transmembrane translocation, proteolytic cleavage required exocytosis synaptic vesicles release...