the purpose of this study was to analyze biotechnology experts’ attitude in university centers of tehran province towards use of transgenic plants. the design of the study was descriptive, and it was carried out through a survey method. the target population consisted of biotechnology experts in university centers of tehran province (n=75). though kerjcie and morgan sample size table and ratio ...

The present study deals with the establishment of rolA-transgenic and rolB-transgenic plants for first time through Agrobacterium tumefaciens mediated transformation, exploiting inherent morphogenic potential an important medicinal plant, Bacopa monnieri (L.) Wettst. showed integration expression rolA rolB genes respectively, whereas Ri-transformed rolA, rolB, rolC rolD genes. Morphogenic diffe...

many recent studies have shown that glycosylation patterns of agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. to achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. to isolate this promoter, pcr with specific primers was perform...

background: plants are among promising and suitable platform systems for production of recombinant biopharmaceutical proteins due to several features such as safety, no need for fermentation, inexpensive investment, and fast and easy scale-up. human insulin is one of the most widely used medicines in the world. up to now different expression systems including escherichia coli, yeast and cho hav...

one concern about using transgenic plants is the genetic variation that occurred from theirs tissue culture and regeneration. molecular markers are an important element for efficient and effective determination of genetic variation. the present work was carried out to assess the genetic uniformity of transgenic cottons (bt and chitinase lines), using rapd, issr molecular markers and sds-page an...

Canola (Brassica napus L.) is an important oilseed crop. A serious problem in cultivation of this crop andyield loss, are due to fungal disease stem rot caused by Sclerotinia sclerotiorum. The pathogenesis-related(PR) proteins have the potential for enhancing resistance against fungal pathogen. Thaumatin-like proteins(TLPs) have been shown to have antifungal activity on variou...

Rose is the most favorite cut flowers all over the world. Production of high-quality flowers, prevention, and delay of flower senescence, is a major goal in floriculture. Now a day, biotechnological approaches have been used to improve ornamental attributes. Tissue culture and genetic transformation appear to offer valuable advancements for operating floral characteristics. In this study, after...

Engineering transcription factors (TF) hold promise in enhancing abiotic stress tolerance plants. In this study, one of the popular rice varieties South India, namely ADT 43, was engineered with a TF AtDREB1A driven by stress-inducible rd29A promoter. PCR and Southern hybridization were employed to confirm integration copy number transgene. Transgenic lines (T1) 43 showed enhanced drought salin...