The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different species, including long non-coding RNAs (lncRNAs). Dense viral can multigenic transcripts in addition to commonly observed antisense transcripts. It is essential study the biological role...

Single-cell RNA sequencing (scRNA-seq) provides rich transcriptomic information for studying molecular events and cell heterogeneity at the single-cell level. However, it is challenging to obtain sequence from rare or low-abundance genes in presence of other highly abundant genes. We report here a CRISPR-Cas9 technique depletion high-abundance transcripts, resulting preferential enrichment tran...

Introduction To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium. Methods: Total RNA was isolated from the human conjunctiva and central cornea. Those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea wer...