Hepatitis B virus (HBV), although a DNA virus, replicates using reverse transcriptase encoded by the HBV polymerase (pol) gene. The biochemical dissection of HBV pol has been hampered by failure to liberate enzymatically active protein from nucleocapsids. Here, we have employed a yeast-based genetic approach to express the HBV reverse transcriptase. In this strategy, the reverse transcriptase o...

Several phytochemicals exhibited high level of antiviral activity. Medicinal plant possessed activity via many mechanisms included inhibition viral replication, the assembly intracellular infectious virus particles, infectivity, RNA polymerase, DNA neuraminidase, protease, reverse transcriptase and protein expression other mechanisms. The current review discuss medicinal plants with their action.

The transcriptase activity of influenza A virus ribonucleoproteins was inhibited by 42 to 49% in vitro in the presence of membrane (M) protein. The addition of M protein to the system of ribonucleoprotein preparations isolated from rimantadine-sensitive or rimantadine-resistant influenza virus strains, as well as the addition of M protein isolated from a sensitive strain, in the presence of rim...

Samples of three nonmalignant and seven leukemic human cells were examined for DNA polymerase activity that could be identified as RNA tumor virus reverse transcriptase. Experiments on virus-infected model animal cells provided the basis for cell fractionation procedures, and reconstituted systems of known virus, added to human cells, established a threshold of virus detection by enzyme assay a...

The HIV replicative cycle reveals several virus-specific events that could function as targets for chemotherapeutic intervention. The compounds that are presently available as anti-HIV drugs are targeted at either the substrate binding site of the reverse transcriptase (zidovudine, didanosine, zalcitabine, stavudine, lamivudine) or a non-substrate binding site of the reverse transcriptase (nevi...

the biological mechanisms of tooth movement are based on the response of periodontal tissues to mechanical forces. the final result of these responses is remodeling of the extracellular matrix. tissue reactions may vary depending upon the type, magnitude and duration of the applied forces. the purpose of the present study was to analyze the effects of centrifugal force on the transcription of c...

background human enteroviruses are members of picornaviridae family; they are non-enveloped, icosahedral viruses with positive rna as genome. echovirus 30 is an important member of enteroviruses that is recognized in outbreaks of enterovirus meningitis. objectives the aim of this study was to determine relative frequency of echovirus 30 as an important agent of aseptic meningitis among children...