The effects of treatment with cortisol on the growth and alanine-a-ketoglutarate transaminase 1 activity of Walker carcinosarcoma 256 were studied. Similarly, the effects of other conditions known to enhance gluconeogenic activity, such as diabetes, fasting, and high protein intake, were investigated. Each of these conditions was previously shown to be associated with elevated activity of alani...

The carbonyl reagent amino-oxyacetate is frequently used in metabolic studies to inhibit individual pyridoxal phosphate enzymes. The reaction of this compound with three such enzymes, aspartate transaminase, 4-aminobutyrate transaminase and dopa (3,4-dihydroxyphenylalanine) decarboxylase, was studied to determine the extent to which the inhibition is reversible and the rates at which it takes p...

objective(s): titanium dioxide (tio2) nanoparticles (nps) are widely used in commercial food additives and cosmetics worldwide. uptake of these nanoparticulate into humans by different routes and may exhibit potential side effects, lags behind the rapid development of nanotechnology. thus, the present study designed to evaluate the toxic effect of mixed rutile and anatase tio2 nps on serum bioc...

In a preliminary report (I), we have described an intense inhibitory action of the sulfate esters of estrone, estradiol, and diethylstilbestrol on the kynurenine transaminase of rat kidney and on the phosphorylase a of rabbit muscle. More recently, we have also found that this interaction results in protection of the kynurenine transaminase from proteolysis and from the inactivation that normal...

Kynurenine transaminase activity in rat liver was found in both the mitochondrial and supernatant fractions. The mitochondrial and supernatant fractions contained (a) kynurenine-pyruvate transaminase, which showed a preference for pyruvate as amino acceptor and a pH optimum between 8.0 and 8.5, and (b) kynurenine-alpha-oxoglutarate transaminase, with a preference for alpha-oxoglutarate and a pH...

Recent studies in vitro of tyrosine metabolism have revealed the distribution and the r&e of the tyrosine-a-ketoglutaric acid transaminase system (l-8). In certain of these studies the obligatory rBle of this transaminase as a first step in the conversion of tyrosine to homogentisic acid has been indicated (6-S). The present study is concerned with the partial purification of dog liver tyrosine...