a palladium composite membrane was prepared via electroless plating technique using organic-inorganic method during activation process. the ceramic support surface was modified by two tio2-boehmite and one γ-alumina layers to avoid pd penetration in support pores. thin and defect-free pd composite membrane was obtained by creating a relative smoothness on the ceramic support and using pd nanopa...

Corn steep water as effluent which arrives in environment from starch processing industry has adverse effects on the environment and also large volume of fresh water is wasted. Corn step water is composed of high concentration of proteins, sugar, starch, vitamin and minerals, so it can be used as substrate for production of biological products. Hence, corn steep water concentration is important...

We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic pathways to the lytic vacuole in tobacco (Nicotiana tabacum) BY-2 cells. Secretory carrier membrane proteins (SCAMPs) are post-Golgi, integral membrane proteins mediating endocytosis in animal cells. To define the endocytic pathway in plants, we cloned the rice (Oryza sativa) ho...

We develop an analytical theory that accounts for the image and surface charge interactions between a charged dielectric membrane and a DNA molecule translocating through the membrane. Translocation events through neutral carbon-based membranes are driven by a competition between the repulsive DNA-image-charge interactions and the attractive coupling between the DNA segments on the trans and th...

In the current research, ABS-co-PEG nanofiltration membrane was prepared by solution casting technique using N, N dimethyl acetamide as solvent. The effect of PEG concentration as additive in the casting solution on membrane flux, salt rejection, phase inversion time, water content, membrane porosity, membrane tensile strength and fouling was studied. Also the eff...

We previously demonstrated, using fluorescence recovery after photobleaching, that clathrin in clathrin-coated pits at the plasma membrane exchanges with free clathrin in the cytosol, suggesting that clathrin-coated pits are dynamic structures. We now investigated whether clathrin at the trans-Golgi network as well as the clathrin adaptors AP2 and AP1 in clathrin-coated pits at the plasma membr...

Dehiscence of median sternotomy wounds remains a clinical problem. Wall forces in thin-walled pressure vessels can be calculated by membrane stress theory. An ellipsoid pressure vessel model of sternal forces is presented together with its application for optimal wire placement in the sternum. Sternal forces were calculated by computational simulation using an ellipsoid chest wall model. Sterna...

SNARE-dependent membrane fusion requires the disassembly of cis-SNARE complexes (formed by SNAREs anchored to one membrane) followed by the assembly of trans-SNARE complexes (SNAREs anchored to two apposed membranes). Although SNARE complex disassembly and assembly might be thought to be opposing reactions, the proteins promoting disassembly (Sec17p/Sec18p) and assembly (the HOPS complex) work ...

We have re-examined the effects of the ionophore monensin on the Golgi apparatus of sycamore maple suspension-cultured cells using a combination of high pressure freezing, immunocytochemical and biochemical techniques. Exposure of the cells to 10 microM monensin, which reduces protein secretion by approximately 90%, resulted first in the swelling of the trans-Golgi network, then of the trans-mo...

Distributions of each amino acid in the trans-membrane domain were calculated as a function of the membrane normal using all currently available alpha-helical membrane protein structures with resolutions better than 4 A. The results were compared with previous sequence- and structure-based analyses. Calculation of the average hydrophobicity along the membrane normal demonstrated that the protei...