Infection with Toxoplasma gondii is often asymptomatic and, when acquired during pregnancy, may lead to connatal toxoplasmosis in the offspring. The newly introduced Vitros anti-Toxoplasma immunoglobulin G (IgG) and IgM assays, designed for the Vitros ECiQ immunodiagnostic system, a fully automated system based on chemiluminescence, were evaluated as a screening method for the serological detec...

A seroprevalence survey of toxoplasmosis among 91 Thai Buddhist monks was conducted in two temples in Nakhon Pathom Province, Thailand. A high prevalence (8.8%) of toxoplasmosis among these subjects was observed. Control of cats and dogs in Buddhist temples should be made a current public health strategy. A total of 91 male Buddhist monks from the two temples were included into this study. Afte...

Cats are the definitive hosts of Toxoplasma gondii. Infected cats excrete oocysts in their feces, infecting humans and other animals. The objective of the present study was to determine the presence of anti-Toxoplasma antibodies in cat owners and their pets, and determine if there was a relationship between Toxoplasma infection and humans who live with infected cats. IgG anti-Toxoplasma antibod...

Variations in the prevalence of antibody to toxoplasma gondii is reported from various countries (Hong Kong 9.8%, London 22%, New York 30%, Paris 84%) and from various cities in the same country(Jeddah 32%, Abha 51%). An area densely populated with cats is believed to be heavily contaminated with toxoplasma oocysts, leading to an increasing infection. As MedinaMunawarah has a large number of ca...

Citation Mashayekhi, Mona, Michelle M. Sandau, Ildiko R. Dunay, Eva M. Frickel, Asis Khan, Romina S. Goldszmid, Alan Sher, et al. “CD8[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 That Controls Acute Infection by Toxoplasma Gondii Tachyzoites.” Immunity 35, no. 2 (August 2011): 249–259. As Published http://dx.doi.org/10.1016/j.immuni.2011.08.008 Publisher Elsevier/Ce...

Based on a serum-free and CO,-independent culture of Toxoplasma go&ii in vitro, a simple single arrangement mass production of trophozoites of the highly virulent BK strain of this parasite was developed. Starting simultaneously, lo7 host cells (HEp-2) and 10’ Toxoplasma trophozoites were cultured in one cell-factory@ for 5 days, removing unattached cells, cell remnants and extracellular parasi...

Mononuclear phagocytes, particularly macrophages (M phi) that have been activated by lymphokines, are the principal defense against intracellular pathogens such as Toxoplasma gondii. To determine reasons for the newborns' susceptibility to Toxoplasma infection, we compared: the interaction of Toxoplasma with newborns' mononuclear phagocytes (blood monocytes and two types of newborn M phi, those...

This article is an attempt to identify the most significant highlights of Toxoplasma research over the last 25 years. It has been a period of enormous progress and the top 25 most significant advances, in the view of this author, are described. These range from the bench to the bedside and represent a tremendous body of work from countless investigators. And, having laid out so much that has be...