The need for safe and effective antifungal agents increases in parallel with the expanding number of immunocompromised patients at risk for invasive fungal infections. The emergence of fungal pathogens resistant to current therapies further compounds the dearth of antifungal agents. Currently available antifungal compounds act on targets also found in mammalian cells (34), which may result in t...

β Cyclodextrin nanocomplexes with extensive whey and colostrum hydrolysates possessing acceptable flavor properties serve as potential sources of bioactive peptides. In this study, comparative characterization dairy protein their complexes cyclodextrin is presented. Antioxidant activity studied samples was estimated by fluorometric method, the formation clathrates cyclic oligosaccharide determi...

mycoplasma genitalium (m.genitalium) is a sexually transmitted pathogen. detection of this microorganism in clinical specimens by culture is rather difficult and time consuming. the aim of this study was to produce polyclonal antibody against a synthetic peptide from p110 protein of m.genitalium in order to develop a diagnostic tool for detection of this microorganism in clinical specimens. a s...

Self-assembly of amyloid β-protein (Aβ) into neurotoxic oligomers and fibrillar aggregates is a key process thought to be the proximal event leading to development of Alzheimer's disease (AD). Therefore, numerous attempts have been made to develop reagents that disrupt this process and prevent the formation of the toxic oligomers and aggregates. An attractive strategy for developing such reagen...

β-Amyloid (Aβ) accumulation in the brain is widely accepted to be critical to the development of Alzheimer's disease (AD). Current efforts at reducing toxic Aβ40 or 42 have largely focused on modulating γ-secretase activity to produce shorter, less toxic Aβ, while attempting to spare other secretase functions. In this paper we provide data that offer the potential for a new approach for the tre...

Objective(s):This study aims at exploring cytotoxic activity of different peptides derived from VSVG protein against MCF-7 and MDA-MB-231 breast cancer cell lines and human embryonic kidney normal cell (HEK 293).    Materials and Methods: The ANTICP web server was used to predict anticancer peptides. The cytotoxic activity of peptides with high score (P26, P7) and low score (P19) was examined b...

α-Cyano-4-hydroxycinnamic acid (CHCA), an organic matrix molecule for matrix-assisted laser desorption/ionization mass spectrometry, was adsorbed to NH4 (+)-type zeolite surface, and this new matrix was used for the detection of low-molecular-weight compounds. It was found that this matrix could simplify the mass spectrum in the low-molecular-weight region and prevent interference from fragment...

Brain amyloid plaques are a hallmark of Alzheimer's disease (AD), and primarily consist of aggregated Aβ peptides. While Aβ 1-40 and Aβ 1-42 are the most abundant, a number of other Aβ peptides have also been identified. Studies have indicated differential toxicity for these various Aβ peptides, but in vivo toxicity has not been systematically tested. To address this issue, we generated improve...

Synthesis of long peptides is generally considered as a challenge to peptide chemists, in addition producing significant amounts toxic waste, such DMF. Here we show that using solvent-less methods, ball milling, enabled the production hexapeptide Boc-(Ala-Phe-Gly)2-OBn at gram scale with high overall yield (77 %, 5 linear steps). This longest chain synthesized mill date, which amino acid sequen...