Background: Because of fast rate of degradation and low mammalian cells toxicity the cypermethrin (CPM) is a wide used insecticide in domestic agriculture and in veterinary medicine applications. The compound exerts its pathological impact by down-regulating the antioxidant status. Therefore, current study was designed to evaluate the protective effect of vitamin E on CPMinduced apoptosis and n...

Viral myocarditis is a moderate disease, but it sometimes causes progressive cardiac disorder. Many different viruses have been considered as the agent of viral myocarditis, but Coxsackievirus of the B group, in particular of the Coxsackievirus B3 (CVB3), is more than fifty percent of cases of viral myocarditis. CVB3 is a positive single-stranded RNA virus and a member of the genus Enterovirus ...

Background: Leukemia inhibitory factor (LIF) is a 45-56 kDa glycoprotein that has an important role in proliferation and embryo implantation. Its effect on oocyte maturation and how to exert the function remained to be elucidated. Methods: Immature mice superovulated with human menopausal gonadotropin and germinal vesicle (GV) oocytes were obtained from ovary 48 hours after. GV oocytes were cul...

The characterization of the follicular hierarchy and the expression of the mRNA for the inhibin/activin subunits was investigated in the follicles of 2 lines of turkey hens selected for over 40 generations for increased egg production (Egg line) or increased body weight (Growth line). The follicular hierarchies of 6 hens from the Egg and Growth lines were characterized in middle (45 wk of age) ...

We investigated the correlation between age and total RNA levels in long-lived and control lines of Drosophila melanogaster. Total RNA was extracted at 10 ages from 1-63 days posteclosion from 3 inbred lines, with replication. Three different methods of RNA quantitation gave highly correlated estimates. Total RNA declined substantially with age, exhibiting a dramatic drop in the first few days ...

In a microarray experiment, messenger RNA samples are oftentimes pooled across subjects out of necessity, or in an effort to reduce the effect of biological variation. A basic problem in such experiments is to estimate the nominal expression levels of a large number of genes. Pooling samples will affect expression estimation, but the exact effects are not yet known as the approach has not been ...

The importance of extra cellular matrix (ECM) in development and function of different cells has been reported but little is known about its role in human endometrial epithelial cells. The aim of the present study was to examine effects of artificial ECM (Matrigel) and progesterone on the function and morphology of human endometrial epithelial cells in vitro. Methods: Endometrial samples were ...

Nucleic acid extraction is an attractive alternative to labor-intensive manual methods. In this report, a few methods of total RNA Extraction from Edible mushroom (Agaricus bisporus) compared. This methods consist AGPC(Acid Guanidinium-Phenol-Chloroform), The method according by Lithium chloride and RNx total RNA isolation Kit (CinnaGen). Best method selected for the preparation of high quality...

objectives the current study aimed to assess relationship between s/co rates and hcv-rna levels in the laboratory to identify hcv viremia in patients with a positive anti-hcv. patients and methods all serum samples were assayed for anti-hcv by elisa method. a total of 265 anti-hcv positive patients were tested for hcv-rna testing by quantitative method using artus hcv rg real-time polymerase ch...