materials and methods a total of 300 day-old broiler chicks (ross 308) divided into three equal groups (1 to 3) .the chicks in group 1 were immunized with killed aiv h9n2. the chicks in group 2 were infected with ai virus subtype h9n2. the chicks in group 3 were kept as controls and did not receive any vaccined or lived virus. chicks sera were collected at day 42 usingrns1-elisa and commercial ...

infection with hepatitis c virus (hcv) is a worldwide problem. among hcv proteins, core antigen (ag), besides its importance for diagnostic application is a prime candidate for component of a vaccine. herein, we report results of studies on production of the hydrophilic domain of core ag (2-122) in native conformation by an arabinose induction system in e.coli and the primary characterization o...

introduction: to assess the salivary composition of proteins and minerals in smokers compared with non-smokers.   materials and methods: in this study we compared the total protein and ca, na, k, mg, pb of whole saliva in two groups of men (28 smokers and 31nonsmokers) aged between 29-41years.   results: fifty-nine participants were evaluated. the mean age was 33.14±5.32 years among smokers and...

Background: Food hypersensitivity to walnut usually results in mild symptoms; however, several cases of anaphylactic reactions to this product have been observed. This study aimed to determine the immunochemical characteristics of the Persian walnut and provide the recombinant form of its main allergen. Materials and Methods: The allergenic proteins of th...

The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) and expressed as a recombinant protein in E. coli BL21 (DE3). An indirect enzyme linked immunosorbent assay (ELISA) was developed using the purified Sip protein as a coating antigen, w...

Rheumatoid factors (RF) are autoantibodies with specificity for the Fc portion of human IgG. Production of RF is a characteristic feature of rheumatoid arthritis (RA) and is detectable in high titer in about 90% of these patients. In this study, we measured total IgM, IgA, IgMRF and IgARF in serum and synovial fluid of 45 RA patients by ELISA and the results were compared with those obtained b...

The nucleocapsid (N) gene of infectious bronchitis virus (IBV) strain X isolated in China was expressed in E. coli and was purified as a recombinant protein. An indirect ELISA assay (N-ELISA) for antibody detection was established using the purified recombinant nucleocapsid protein. Antigen coating conditions and serum dilution for the N-ELISA were optimized. The S/P ratio of the absorbency val...

The DMA (DM ? chain) gene, a member of non-classical MHC class II plays an important role in the process presenting antigen peptides by producing DM protein. This study aimed to identify polymorphism gene and their associations with disease resistance traits such as total IgY concentration ND antibody titers IPB-D2 chicken line. sample used was 101 chickens consisted 81 G0 (53 female 28 male) 2...

abstractbackground and objectives: the cultivated tobacco (nicotiana tabacum) is a tropical and annual plant, consisting of a number of species or hybrids belonging to the solanaceae family. bio-chemical reaction to viral infection is different for various plants .one of the most important defensive mechanisms of the host plant against pathogen agents, is biochemical defense and their related c...