Capabilities of mass spectrometry for the analysis of intact proteins can be increased through separation methods. Flow field-flow fractionation (FlFFF) is characterized by the particularly "soft" separation mechanism, which is ideally suited to maintain the native structure of intact proteins. This work describes the original on-line coupling between hollow-fiber FlFFF (HF FlFFF), the microcol...

To further optimize the culturing of preimplantation embryos, we undertook metabolomic analysis of relevant culture media using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). We detected 28 metabolites: 23 embryo-excreted metabolites including 16 amino acids and 5 media-derived metabolites (e.g., octanoate, a medium-chain fatty acid (MCFA)). Due to the lack of informatio...

Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identification of species. Current demand in microbial identification is how to compare unknown strains to the known one quickly, semi-automatically and accurately. In this paper, we present a software tool that allows flexibly microbial matching in a user-f...

A new method is presented for the identification of oligosaccharides obtained by enzymatic digestion of hyaluronan (HA) with bacterial hyaluronidase (E.C. 4.2.2.1, from Streptomyces hyalurolyticus) using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOFMS). Mixtures containing HA oligosaccharides of tetrasaccharide (4-mer)-34-mer were analyzed using this me...

Sequencing of N-terminally blocked proteins/peptides is a challenge since these molecules inhibit processing by Edman degradation. By using high accuracy Fourier transform ion cyclotron resonance (FTICR) tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS), the primary structures of two novel N-terminally blocked antifungal pept...

Analysis of MCPD esters and glycidyl esters in vegetable oils using the indirect method proposed by the DGF gave inconsistent results when salting out conditions were varied. Subsequent investigation showed that the method was destroying and reforming MCPD during the analysis. An LC time of flight MS method was developed for direct analysis of both MCPD esters and glycidyl esters in vegetable o...

Gas chromatography/resonance-enhanced multiphoton ionization/time-of-flight mass spectrometry (GC/REMPI-TOFMS) using an ultraviolet nanosecond laser was employed in the trace analysis of polycyclic aromatic hydrocarbons (PAHs). A standard sample that contained 16 PAHs on the priority list of the Environmental Protection Agency of the United States of America (U.S. EPA) was measured. A sample of...

An efficient and environmentally friendly procedure for one-pot synthesis of 13-acetyl-9-methyl-11-ox-8-oxa-10,12-diazatricyclo [7.3.1. ] trideca-2,4,6-triene from salicylaldehyde, acetylaceton and urea via Biginelli condensation and intramolecular Michael-addition by using magnesium bromide as an expensive and easily available catalyst under solvent-free condition is desired. The structural el...

Monitoring the illegal trade of wool fibres wild vicuña (Vicugna vicugna) and guanaco (Lama guanicoe) is highly desirable. The high market value fleece from these camelid species poses a threat to their populations. A previous study showed that direct analysis in real time time-of-flight mass spectrometry (DART-TOFMS) effectively identifies species. Producing high-resolution data short period m...

Distinct chemical fingerprints of the wood Afzelia pachyloba and A. bipindensis demonstrated an effective method for identifying these two commercially important species. Direct analysis in real-time (DART) time-of-flight mass spectrometry (TOFMS) allowed high-throughput examination chemotypes with vast potential taxonomic, ecological, forensic research wood. is a genus valuable tropical timber...