BACKGROUND Living tissues contain a range of intrinsic fluorophores and sources of second harmonic generation which provide contrast that can be exploited for fresh tissue imaging. Microscopic imaging of fresh tissue samples can circumvent the cost and time associated with conventional histology. Further, intrinsic contrast can provide rich information about a tissue's composition, structure an...

A time-domain numerical code (the so-called Texas code) that solves the Khokhlov-Zabolotskaya-Kuznetsov (KZK) equation has been extended from an axis-symmetric coordinate system to a three-dimensional (3D) Cartesian coordinate system. The code accounts for diffraction (in the parabolic approximation), nonlinearity and absorption and dispersion associated with thermoviscous and relaxation proces...

Nonlinear microscopy has proven to be essential for neuroscience investigations of thick tissue preparations. However, the optical recording of fast (approximately 1 msec) cellular electrical activity has never until now been successfully combined with this imaging modality. Through the use of second-harmonic generation microscopy of primary Aplysia neurons in culture labeled with 4-[4-(dihexyl...

conclusions we did not find significant differences in ventricular function or ventricular septal thickness in the fetuses of mothers who were referred to our hospital with a previous diagnosis of diabetes mellitus compared with the fetuses of non-diabetic mothers. this could be due to early identification, appropriate treatment, and tightly controlled diabetes in our study population. it shoul...

conclusion our preliminary results have shown that although there is some overlap between benign and malignant tumors, adding dwi, mr imaging to routine soft tissue tumor protocols may improve diagnostic accuracy. results there was only one patient with a malignant cystic tumor and was not included in the statistical analysis. the median adc values of benign and malignant tumors were 2.31 ± 1.2...

We have developed a nonlinear adaptive optics microscope utilizing a deformable membrane mirror (DMM) and demonstrated its use in compensating for system- and sample-induced aberrations. The optimum shape of the DMM was determined with a random search algorithm optimizing on either two photon fluorescence or second harmonic signals as merit factors. We present here several strategies to overcom...

Currently there is no image-guided modality which is sufficient and exact enough to evaluate the perfusion of tissue in its different layers after transplantation of microvascular flaps. Examination of capillary filling after compression (refill) allows merely a vague estimation. The cutaneous blood flow can be shown with different methods of fluoroscopy, but the perfusion of deeper tissue laye...