Design of organic–inorganic hybrids by anchoring plasmonic materials such as gold nanoparticles (AuNPs) on self-assembled organic substrates is useful but challenging. Herein, in situ the surface a designed spherical assembly via AuS bond formation presented. First, thiol tailed pyrene derivative (2) undergoes solvent dependent self-assembly, transforming into an aggregate (2agg). The (-SH) ri...

Cyanylation of myosin with 6-thiol’4Clcyanato-9-/3-o-ribofuranosylpurine shows a biphasic effect. During the incorporation of the first 4 mol of cyanide the Mg”+-dependent ATPase activity increases approximately &fold, the Ca*+ activity rises only slightly, and the K+(EDTA) activity falls to approximately 5% of its original value. These enzyme preparations can still be inactivated by N-ethylmal...

We have shown [l] that the intensity of band 4 (mol. wt 3 1 000, nomenclature from [2]) of oligomycin-sensitive ATPase from beef heart mitochondria increases in the presence of thiol reagent, 2-mercaptopropionylglycine. Here we show that the intensity of this band (which is lacking in oligomycin-insensitive ATPase [3]) decreases in the presence of 0.2 MM of the uncoupler carbonylcyanide-p-trifl...

Numerous investigations have shown that 70-kDa heat shock protein (Hsp70) homologs interact tightly with hydrophobic proteins and functionally assist proteins in membranous organelles and environments. One such protein is the Chlamydia trachomatis Hsp70 that is associated with isolated outer membrane complexes of infectious elementary bodies (EB). Previous observations have indicated that chlam...

Objective: The objectives of the present work were to carry out thiol-modification (i.e., thiolation) fenugreek polysaccharide (FP) and assess synthesized thiolated product (TFP) as a mucoadhesive excipient in designing formulations (metronidazole gels metronidazole buccal discs). Methods: Extracted CG was thiol-modified via an esterification reaction by utilizing thioglycolic acid with acidic ...

A range of mitochondria-targeted probe molecules that comprise a lipophilic cation covalently attached to an active moiety have been developed. The lipophilic cation causes the accumulation of these molecules into mitochondria, driven by the mitochondrial membrane potential. To date, we have targeted antioxidants, spin traps, thiol reagents and DNA-alkylating compounds to mitochondria. The sele...

Cyanylation of myosin with 6-thiol’4Clcyanato-9-/3-o-ribofuranosylpurine shows a biphasic effect. During the incorporation of the first 4 mol of cyanide the Mg”+-dependent ATPase activity increases approximately &fold, the Ca*+ activity rises only slightly, and the K+(EDTA) activity falls to approximately 5% of its original value. These enzyme preparations can still be inactivated by N-ethylmal...

OBJECTIVES To develop a novel and automated assay determining plasma thiol/disulphide homeostasis, which consists of thiol-disulphide exchanges. DESIGN AND METHODS Native thiol and total thiol concentrations were synchronously measured as a paired test. In the first vessel, the amount of native thiol groups was measured by a modified Ellman reagent. At the parallel run, first, dynamic disulph...

The activity of the nuclear factor I/CCAAT transcription factor (NFI/CTF) is negatively regulated by oxidative stress. The addition of relatively high (millimolar) H(2)O(2) concentrations inactivates cellular NFI DNA-binding activity whereas lower concentrations can repress NFI/CTF transactivating function. We have investigated the mechanism of this regulation using Gal4 fusion proteins and tra...