An antiserum raised against whole cells of Thiobacillus ferrooxidans was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of T. ferrooxidans in the microbial ...

Ferrous sulphate, a waste product in titanium white production at the Chemical Plant "Police" S.A., was subjected to microbiological oxidation with Thiobacillus ferrooxidans bacteria. The kinetic parameters of the culture growth were calculated on the basis of the Monod equation. The process was found to be best described by the model of the autocatalytic 1st order reaction with respect to the ...

With the financial protection of National Iranian Copper Industries Company, the composition of bacterial populations in Aliabad copper mine was investigated by electron microscopy and by analysis of DNA obtained from bacteria after ores laboratory cultures. This analysis consisted of the characterization of the spacer regions between the 16 and 23s genes in the bacterial rRNA genetic loci afte...

In a study of chemosynthesis (the fixation of CO(2) by autotrophic bacteria in the dark) in Thiobacillus thiooxidans, the data obtained support the following conclusions: 1. CO(2) can be fixed by "resting cells" of Thiobacillus thiooxidans; the fixation is not "growth bound." 2. The physiological condition of the cell is of considerable importance in determining CO(2) fixation. 3. CO(2) fixatio...

Analysis of phylogenomic metrics of a recently released draft genome sequence of the halotolerant, acidophile 'Thiobacillus prosperus' DSM 5130 indicates that it is not a member of the genus Thiobacillus within the class Betaproteobacteria as originally proposed. Based on data from 16S rRNA gene phylogeny, and analyses of multiprotein phylogeny and average nucleotide identity (ANI), we show tha...

The complete 5S and 16S ribosomal DNA (rDNA) sequences of the facultatively chemolithotrophic bacterium Thiobacillus cuprinus and results of a comparison of these sequences with homologous sequences from several proteobacterial species supported affiliation of T. cuprinus with the beta 1 subgroup of the Proteobacteria. T. cuprinus, Thiobacillus intermedius, Thiobacillus perometabolis, and Thiob...

It is shown that there exists in the autotrophic bacterium Thiobacillus thiooxidans a measurable oxygen uptake in the absence of the specific nutrient (sulfur). This respiration is shown to be due to the utilization of organic materials which must have been previously synthesized by the chemosynthetic process, providing evidence that autotrophic bacteria contain a dissimilatory process which in...

Nitrogen pollution in wastewater has been considered a worldwide risk to ecosystems. Bioelectrochemical technologies have developed recently remove nitrogen. In addition nitrogen, microplastics, as emergency pollutants wastewater, could potentially affect nitrogen removal efficiency due their toxic effects on the activity of microorganisms. This study explored how microplastics would influence ...

considering high alkalinity of iranian soils and lack of availability of some nutrients in those conditions, sulfur consumption is one approach to increase the availability of insoluble nutrients especially in calcareous and alkaline soils. effectiveness of sulfur depends on activity of sulfur oxidizing bacteria especially thiobacillus genus (t). in order to study the qualitative yield and morp...