نتایج جستجو برای: theileria equi
تعداد نتایج: 4514 فیلتر نتایج به سال:
The study was aimed at determining the cause of the diseases in three horses exhibiting symptoms of fever, ataxia, mucus membrane paleness, haematuria and thrombocytopenia. The PCR technique revealed the presence in the blood of 18S RNA Babesia/Theileria spp. genetic material. DNA amplification using primers RLB F2 and RLB R2 produced 430 bp size products. The sequences of these PCR products de...
Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...
background: equine piroplasmosis is caused by two haemoprotozoan parasites: babesia caballi and theileria equi . negative economic impact on international trade has been associated to endemic sites. this is the reason why carrier detection requires reliable diagnostic methods. various diagnostic modalities can be used alone or in combination including pcr. however, genetic variation of commonly...
A quantitative real-time polymerase chain reaction (qPCR) assay using a TaqMan minor groove binder (MGB) probe was developed for the detection of Babesia caballi infection in equids from South Africa. Nine previously published sequences of the V4 hypervariable region of the B. caballi 18S rRNA gene were used to design primers and probes to target unique, conserved regions. The B. caballi TaqMan...
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