the purpose of this study was to examine the potential application of sorbitol, sucrose and gelatin in combination with glycerol in order to theileria annulata infected cell line cryo-preservation. the efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. as a result, the...

The apicomplexan parasite, Theileria annulata, is the causative agent of tropical theileriosis, a devastating lymphoproliferative disease of cattle. The schizont stage transforms bovine leukocytes and provides an intriguing model to study host/pathogen interactions. The genome of T. annulata has been sequenced and transcriptomic data are rapidly accumulating. In contrast, little is known about ...

The tick-borne protozoan parasite Theileria annulata causes tropical theileriosis, a severe disease of cattle that occurs across the Mediterranean littoral, the Middle East and Southern Asia. In the Mediterranean region, the disease has long been perceived as being a constraint to livestock production in North Africa and Turkey but was believed to have minimal impact in Southern European countr...

The multinucleated macroschizont stage of the protozoon Theileria annulata is an intracellular parasite of bovine leukocytes. The parasite induces the host cell to proliferate, and divides in synchrony with the immortalised host cell. Differentiation to the next stage occurs within the host cell culminating in the release of merozoites and destruction of the leukocyte. In this study clones of T...

background: although camels represent a valuable source of food, wool and hide in many countries, in-depth information about their vector-borne pathogens is scarce compared to other animals. the aim of the current study was to characterize vector-borne protozoa in the blood of dromedaries from iran by molecular tools. methods: from june to july 2014, 200 peripheral blood samples were collected ...

This study was conducted to measure selected haematological parameters in Holstein cattle naturally infected with Theileria annulata. Haematological analysis indicated significant decreases in red blood cell count, haematocrit value, haemoglobin amount, mean corpuscular haemoglobin concentration, and white blood cell, lymphocyte, neutrophil, monocyte, eosinophil, and basophil counts. On the oth...

The present study was carried out in Circeo National Park (Lazio region, Central Italy), in order to collect data about mosquito (Diptera, Culicidae) fauna in a protected area for biodiversity. From 2003 to 2004 seasonal surveys allowed to collect and to identify 380 larvae and 713 adult mosquitoes in 6 sites. A total of 15 mosquito species belonging to 6 genera were recorded; the most abundant...

Hyaloma spp. has been described as the most abundant species that transmitted ovine piroplasms in Fars province. The traditional methods of diagnosis infected ticks vectors are based on morphological characteristics that have some problems. The best methods for detection and characterization of the protozoan parasites in ticks are the molecular biological methods. The aim of this study was to s...

Theileria parasites invade and transform bovine leukocytes causing either East Coast fever (T. parva), or tropical theileriosis (T. annulata). Susceptible animals usually die within weeks of infection, but indigenous infected cattle show markedly reduced pathology, suggesting that host genetic factors may cause disease susceptibility. Attenuated live vaccines are widely used to control tropical...