Template-free polymorph discrimination of CaCO(3) can be easily realized by a so-called double-jet method in aqueous solution under mild conditions, which may shed new light on the controlled crystallization of other inorganic material systems in which polymorph selection is of critical importance or is highly relevant to their properties.

Current methodologies for arraying proteins using cell-free protein synthesis on a chip have spatial limitations that prevent reaching ultra-high density necessary for high throughput analysis. To circumvent this, we developed an on-chip method based on microcompartmentalization of protein synthesis. Proteins are synthesized in arrayed micrometer scale chambers from confined DNA template molecu...

Chloramphenicol can inhibit protein synthesis in mammalian cell-free systems as effectively as it inhibits protein synthesis in analogous microbial systems. Significant inhibition in mammalian systems was obtained only when protein synthesis was stimulated by the addition of template RNA to the system, there being comparatively little inhibition of protein synthesis by ribosomes in the absence ...

RNA template-polymerase complex, influenza virus, complementary RNA (synthesis in vitro) The enzyme-template complex of influenza RNA polymerase (fowl plague virus) was purified 200-fold. The sole virus component found in this preparation was RNP-antigen. All attempts to remove the internal template led to an irreversible loss of enzyme activity. The complex was essentially free of nucleases. I...

In the synthesis of zeolites and related crystalline materials with open-frameworks, a single structure is obtained in the presence of many different templates, known as the "one-structure/multiple-templates" phenomenon. However, the reasons behind this phenomenon have yet to be elucidated. By analyzing the possible starting point of crystallization in several "one-structure/multiple-templates"...

Regulation of argA operon expression in Escherichia coli K-12 was studied in a cell-free, deoxyribonucleic acid-dependent, enzyme-synthesizing system. lambdaAZ-7 deoxyribonucleic acid, which carries a fusion of the lacZ structural gene to the argA operon so that beta-galactosidase synthesis is under argA regulation, was used as the template. To eliminate extraneous readthrough from lambda promo...

Imprinted nanoparticles present several advantages respect to bulk imprinted materials, but, when prepared by traditional methods, their usefulness is limited as the approaches are costly or require complex optimization steps, while purification from template molecules challenging. An innovative approach solid-phase synthesis. It consists in covalent immobilization of onto a solid support, poly...

Cereal yellow dwarf virus (CYDV) RNA has a 5'-terminal genome-linked protein (VPg). We have expressed the VPg region of the CYDV genome in bacteria and used the purified protein (bVPg) to raise an antiserum which was able to detect free VPg in extracts of CYDV-infected oat plants. A template-dependent RNA-dependent RNA polymerase (RdRp) has been produced from a CYDV membrane-bound RNA polymeras...

Replicative DNA polymerases generally cannot pass lesions in the template strand. Now there is accumulating evidence for the widespread existence of a separate class of DNA polymerases that can carry out translesion synthesis in both mutagenic and error-free ways.

oxide nanoparticles can exhibit unique physical and chemical properties due to their limited size and a high density of corner or edge surface sites. in this study, mgo nanoparticle was synthesized using mg(ch3coo)2 and hexamethylenetetramine as starting materials. the structure and optical properties of these particles are investigated by using x-ray diffraction (xrd), scanning electron micros...