O presente artigo tem por objetivo compreender as relações afetivo-sexuais de homens gays produzidas em um terreiro amazônico, zona bragantina, no Pará. Deste modo, experiências sociais que são constituídas potencializam vivências destes fogem à heterossexualidade, ao passo modificam terreiro. Sendo assim, e etnografia acompanha o trajeto da pesquisa entrevistas semiestruturadas com os também f...

Engineering novel allostery into existing proteins is a challenging endeavor to obtain novel sensors, therapeutic proteins, or modulate metabolic and cellular processes. The RG13 protein achieves such allostery by inserting a circularly permuted TEM-1 β-lactamase gene into the maltose binding protein (MBP). RG13 is positively regulated by maltose yet is, serendipitously, inhibited by Zn(2+) at ...

The novel beta-lactamase CTX-1 (pI 6.3) encoded on a transferable 84-kilobase plasmid was found in six different bacterial species. It was responsible for a significant decrease in susceptibility towards most penicillins and cephalosporins, except imipenem, temocillin, and cephalosporins which have a 7-alpha-methoxy substituent. Synergy between either ampicillin, piperacillin, cefotaxime, cefta...

This study evaluates the occurrence of bacteriophages carrying antibiotic resistance genes in animal environments. bla(TEM), bla(CTX-M) (clusters 1 and 9), and mecA were quantified by quantitative PCR in 71 phage DNA samples from pigs, poultry, and cattle fecal wastes. Densities of 3 to 4 log(10) gene copies (GC) of bla(TEM), 2 to 3 log(10) GC of bla(CTX-M), and 1 to 3 log(10) GC of mecA per mi...

This work focuses on the visualization and quantification of Super-paramagnetic iron oxide nanoparticles (SPIO-NPs) inside macrophages using transmission electron microscope (TEM) images. Macrophages have been extensively used as probes for cell and subcellular structure labeling, as well as for drug and gene delivery. In order to track the macrophages movement inside the body, SPIO-NPs are int...

In a survey of resistance to amoxicillin among clinical isolates of Proteus mirabilis, 10 TEM-type beta-lactamases were characterized: (i) the well-known penicillinases TEM-1 and TEM-2, the extended-spectrum beta-lactamases (ESBLs) TEM-3 and TEM-24, and the inhibitor-resistant TEM (IRT) TEM-44 and (ii) five novel enzymes, a penicillinase TEM-57 similar to TEM-1, an ESBL TEM-66 similar to TEM-3,...

cobalt oxide (co3o4) nanoparticleshave been synthesized by hydrothermal method using mixture of cobalt(ii)chloride, triton x-100 and koh in an autoclave at 180 °c for 6 h followed by heating at 400 ºc for 3 h in air. the product have been characterized by fourier transform infrared (ft-ir), uv-vis spectroscopy, powder x-ray diffraction (xrd), scanning electron microscopy (sem) and transmission ...