نتایج جستجو برای: tat

تعداد نتایج: 6516  

Journal: :Cell 2014
Agnieszka Szyk Alexandra M. Deaconescu Jeffrey Spector Benjamin Goodman Max L. Valenstein Natasza E. Ziolkowska Vasilisa Kormendi Nikolaus Grigorieff Antonina Roll-Mecak

Acetylation of α-tubulin Lys40 by tubulin acetyltransferase (TAT) is the only known posttranslational modification in the microtubule lumen. It marks stable microtubules and is required for polarity establishment and directional migration. Here, we elucidate the mechanistic underpinnings for TAT activity and its preference for microtubules with slow turnover. 1.35 Å TAT cocrystal structures wit...

Journal: :Journal of virology 2003
Ilia Tikhonov Tracy J Ruckwardt Glen S Hatfield C David Pauza

The human immunodeficiency virus Tat protein is essential for virus replication and is a candidate vaccine antigen. Macaques immunized with Tat or chemically modified Tat toxoid having the same clade B sequence developed strong antibody responses. We compared these antisera for their abilities to recognize diverse Tat sequences. An overlapping peptide array covering three clade B and two clade ...

Journal: :Jurnal Sain Veteriner 2023

Virus Penyakit Jembrana adalah virus yang menyebabkan jembrana pada sapi bali. Vaksin protein rekombinan dibuat dengan memasukkan DNA atau gen mengkode imunogenik ke dalam vektor ekspresi prokariotik sehingga dapat mengekspresikan akan dijadikan vaksin. Salah satu struktural penyakit tat digunakan sebagai vaksin rekombinan. Tujuan dari penelitian ini untuk mengkloning pET15b tat. Konfirmasi pET...

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2000
M Rusnati G Taraboletti C Urbinati G Tulipano R Giuliani M P Molinari-Tosatti B Sennino M Giacca M Tyagi A Albini D Noonan R Giavazzi M Presta

Tat protein, a trans-activating factor of the human immunodeficiency virus type 1, acts also as an extracellular molecule modulating gene expression, cell survival, growth, transformation, and angiogenesis. Here we demonstrate that human thrombospondin-1 (TSP), a plasma glycoprotein and constituent of the extracellular matrix, binds to glutathione-S-transferase (GST)-Tat protein but not to GST....

Journal: :Journal of virology 2003
Baode Xie Mark A Wainberg Alan D Frankel

Human immunodeficiency viruses (HIVs) and the related bovine lentiviruses bovine immunodeficiency virus (BIV) and Jembrana disease virus (JDV) utilize the viral Tat protein to activate viral transcription. The arginine-rich RNA-binding domains of the Tat proteins bind to their cognate transactivation response element (TAR) RNA hairpins located at the 5' ends of the viral mRNAs, resulting in enh...

Journal: :Journal of virology 1999
P D Bieniasz T A Grdina H P Bogerd B R Cullen

The biological activity of the human immunodeficiency virus type 1 (HIV-1) Tat (Tat1) transcriptional activator requires the recruitment of a Tat1-CyclinT1 (CycT1) complex to the TAR RNA target encoded within the viral long terminal repeat (LTR). While other primate immunodeficiency viruses, such as HIV-2 and mandrill simian immunodeficiency virus (SIVmnd), also encode Tat proteins that activat...

2016
María Rosa López-Huertas Jasmine Li Anjum Zafar Sara Rodríguez-Mora Carlota García-Domínguez Elena Mateos José Alcamí Sudha Rao Mayte Coiras

PKCθ is essential for the activation of CD4(+) T cells. Upon TCR/CD28 stimulation, PKCθ is phosphorylated and migrates to the immunological synapse, inducing the activation of cellular transcription factors such as NF-κB and kinases as ERK that are critical for HIV-1 replication. We previously demonstrated that PKCθ is also necessary for HIV-1 replication but the precise mechanism is unknown. E...

Journal: :Journal of immunology 2009
Emanuele Fanales-Belasio Sonia Moretti Valeria Fiorelli Antonella Tripiciano Maria R Pavone Cossut Arianna Scoglio Barbara Collacchi Filomena Nappi Iole Macchia Stefania Bellino Vittorio Francavilla Antonella Caputo Giovanni Barillari Mauro Magnani Maria Elena Laguardia Aurelio Cafaro Fausto Titti Paolo Monini Fabrizio Ensoli Barbara Ensoli

Tat is an early regulatory protein that plays a major role in human HIV-1 replication and AIDS pathogenesis, and therefore, it represents a key target for the host immune response. In natural infection, however, Abs against Tat are produced only by a small fraction (approximately 20%) of asymptomatic individuals and are rarely seen in progressors, suggesting that Tat may possess properties dive...

Journal: :Intervirology 2007
Lin Zheng Yida Yang Lu Guocai C David Pauza Maria S Salvato

OBJECTIVE To investigate the effect of HIV Tat protein on Bcl-2 expression in human monocytes, and observe apoptosis of Tat-stimulated monocytes induced by TNF-alpha-related apoptosis-induced ligand (TRAIL). METHODS Western blot was used to detect Bcl-2 expression in monocytes stimulated by HIV Tat protein, and Annexin V and 7-AAD staining were used to detect apoptosis of monocytes induced by...

Journal: :Blood 2005
Serena Marchiò Massimo Alfano Luca Primo Daniela Gramaglia Luca Butini Luisa Gennero Enrico De Vivo Wadih Arap Mauro Giacca Renata Pasqualini Federico Bussolino

Human immunodeficiency virus-1 (HIV-1) Tat, a nuclear transactivator of viral gene expression, has the unusual property of being released by infected cells. Recent studies suggest that extracellular Tat is partially sequestered by heparan sulfate proteoglycans. As a consequence, Tat is concentrated on the cell surface and protected from proteolytic degradation, thus remaining in a biologically ...

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