Protoplasts were isolated from Trichoderma harzianum strain PTh18 using lysing enzymes and self-fusion of T. harzianum protoplasts was carried out using polyethylene glycol in STC buffer. The fused protoplasts of T. harzianum were regenerated and 15 self-fusants were selected to study the chitinase production and biocontrol activity. High chitinase activity was measured in the culture filtrates...

Root rot disease of Cuminum cyminum caused by Fusarium solani is one the most destructive diseases threatening cumin production. The present study investigates biocontrol potential some endophytes against F. and their effect on induction defense-related enzymes in a greenhouse. results herein presented illustrate strong three (out twelve) endophytes. During vitro assay, isolates demonstrated my...

strains of trichoderma harzianum s. l. have a great importance because of affecting mankind by being used as biological control agents against plant pathogens. in the present study, we planned to characterize the phylogenetic structure of t. harzianum recovered from different geographical origins and various habitats in some climates of iran (nc, scd, csw). in order to screen 239 collected tric...

A laboratory and green house experiment was carried out on the comparative antagonistic performance of four different bioagents (Aspergillus sp., Gliocladium virens, Trichoderma harzianum and T. viride) isolated from soil against Rhizoctonia solani. Under laboratory conditions, T. harzianum exhibited maximum (75.55%) mycelial growth inhibition of R. solani This was followed by T. viride, which ...

Employing Illumina Hiseq whole genome metagenome sequencing approach, we studied the impact of Trichoderma harzianum on altering the microbial community and its functional dynamics in the rhizhosphere soil of black pepper (Piper nigrum L.). The metagenomic datasets from the rhizosphere with (treatment) and without (control) T. harzianum inoculation were annotated using dual approach, i.e., stan...

Although a wide application of pesticides and herbicides are an essential part of augmenting crop yields, excessive use of these chemicals leads to the microbial imbalance of soil, environmental pollution and health hazards to human and animals. An ideal pesticide should have the ability to destroy target pests quickly and should get degrade the toxic and nontoxic substances as quickly as possi...

The investigation was undertaken to enhance the decomposition process by pre-treatment of rice stubble, having higher concentration of lignin. Air-dried rice stubble was treated with 1.8 liter of 1% NaOH and autoclaved. Six cellulolytic fungi, Trichoderma harzianum, Penicillium citrinum, Curvularia lunata, Aspergillus flavus and Alternaria alternata were grown in basal synthetic medium along wi...

The aim of this study was to develop a protocol for the production of fungal bio-pesticides with high efficiency, low cost, and non-polluting fermentation, while also increasing their survival rate under field conditions. This is the first study to develop biocontrol Trichoderma harzianum transformants TS1 that are resistant to benzimidazole fungicides. Agricultural corn stover and wheat bran w...