To achieve large-scale low-cost ex vitro acclimatization of Siberian ginseng plants, heart- and torpedo-shaped secondary somatic embryos (SEs) induced from germinated SEs on agar medium were collected and then inoculated to 10-l bubble column bioreactor, respectively. For plantlet conversion, inoculation of torpedo-shaped secondary SEs was more effective than heart-shaped SEs. TS2 (culture of t...

Shoot-tip cultures of Pyrus elaeagrifolia Pallas, an important gene source for drought and chlorosis resistance in pear rootstock breeding, were established from a wild mature tree originated from seed. Murashige and Skoog basal medium supplemented with different concentrations of benzyladenine (BA) singly or in combination with auxin was used in the study. In the initial culture, the highest p...

The Grateful Dead is a rock and roll band formed in 1965. For thirty years the Grateful Dead have attracted a following of faithful fans, numbering in the hundreds of thousands, known as Deadheads. The lead singer, gUitarist, composer and figurehead of the band was Jerry Garcia. Beginning in the late 1960s, a community grew around the band. To some Deadheads, Jerry represented a father, grandfa...

Bactericidal testing historically has exhibited variable reproducibility, even when prior standardized methods were employed. Several modifications to the National Committee for Clinical Laboratory Standards (NCCLS) broth macrodilution method are proposed to improve reproducibility. Recommended changes from the approved NCCLS guidelines (M21-A and M26-A) include omitting serum supplementation o...

To determine whether acridine orange (AO) staining of blood cultures could be used as a substitute for blind subculture when used in conjunction with the BACTEC system (Johnston Laboratories, Inc., Towson, Md.), the two methods were compared on all BACTEC-negative specimens. Since blind subcultures were routinely performed in our laboratory on days 2 and 6 of incubation, AO staining was also pe...

The criminal subculture of people sentenced to prison produces negative effects in the environment, including segregation and social stratification convicts, physical mental abuse certain categories prisoners, resistance positive resocialization actions, etc. Both theory practice have already demonstrated that destruction subcultures cannot be achieved without attracting other more serious cons...

Despite the criticisms of subcultural theory as a framework for the sociological study of the relationship between youth, music, style and identity, the term ‘subculture’ continues to be widely used in such work. It is a central contention of this article that, as with subcultural theory, the concept of ‘subculture’ is unworkable as an objective analytical tool in sociological work on youth, mu...