Little is known regarding molecular mechanisms involved in sex determination and differentiation in sturgeon species. We addressed this knowledge gap by using next generation pyrosequencing technology to provide transcript libraries and species-specific sequences for mature gonads of shovelnose sturgeon, Scaphirhynchus platorynchus. We then mined these libraries to identify gender-specific tran...

Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10°C, -15°C, -20°C, -30°C and -40°C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/1...

Characteristics of the in vitro propagation of the white sturgeon iridovirus (WSIV) were examined in 6 sturgeon cell lines. One new cell line originating from gonadal tissues (WSGO) produced up to 12-fold more WSIV [approximately 22 TCID50 (50% tissue culture infective dose) cell-1], than that of a previously established reference spleen cell line (WSS-2). Infected WSGO cell cultures were exami...

TheRussian sturgeon,Acipensergueldenstaedtii, is closely related to three other sturgeon species (A. persicus, A. naccarii and A. baerii), with populations in the Caspian Sea containing a cryptic lineage with an A. baerii-like mtDNA profile. Using morphological evidence (morphometrics, meristics) and additional genetic analysis (cytochrome b gene and control region sequencing), cryptic lineages...

The influence of nanoparticles (NPs) on aquatic environments is still poorly documented. The aim of the study was to determine the effects of silver (AgNPs) and copper (CuNPs) nanoparticles on larval Siberian sturgeon (Acipenser baerii) after 21 days of exposure. Acute toxicity of AgNPs on Siberian sturgeon was investigated in a 96-h static renewal study and compared with the toxicity of CuNPs....

Enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) and lipid peroxidation were determined in different tissues (gills, heart, digestive tract, liver, white muscle, skin, red blood cells, swimbladder) of trout (Oncorhynchus mykiss) and sturgeon (Acipenser naccarii). Total tissue antioxidant activity was, except for catalase, generally...

A phylogenetic hypothesis is presented illustrating relationships among 23 extant sturgeon species based on nucleotide data from five mitochondrial genes (cytochrome b, NADH5, control region, 16S, and 12S). The tree supports our previous hypotheses regarding relationships among species of Acipenser and Huso. The introduction of Scaphirhynchus and Pseudoscaphirhynchus to the analysis does not ch...

Scott Sturgeon has claimed to undermine the principal argument for Physicalism, in his words, the view that “actuality is exhausted by physical reality” (Sturgeon 1998, p. 410). In noting that actuality is exhausted by physical reality, the Physicalist is not claiming that all that there is in actuality are those things identified by physics. Rather the thought is that actuality is made up of a...

Sturgeon (order Acipenserformes) provide an ideal taxonomic context for examination of genome duplication events. Multiple levels of ploidy exist among these fish. In a novel microsatellite approach, data from 962 fish from 20 sturgeon species were used for analysis of ploidy in sturgeon. Allele numbers in a sample of individuals were assessed at six microsatellite loci. Species with approximat...

We report the isolation of a novel vitamin K-dependent protein from the calcified cartilage of Adriatic sturgeon (Acipenser nacarii). This 10.2-kDa secreted protein contains 16 gamma-carboxyglutamic acid (Gla) residues in its 74-residue sequence, the highest Gla percent of any known protein, and we have therefore termed it Gla-rich protein (GRP). GRP has a high charge density (36 negative+16 po...