Streptomycetes are mycelium-forming bacteria that produce two thirds of the clinically relevant secondary metabolites. Despite the fact that secondary metabolite production is activated at specific developmental stages of the Streptomyces spp. life cycle, different streptomycetes show different behaviors, and fermentation conditions need to be optimized for each specific strain and secondary me...

Human life expectancy is rapidly increasing with an associated increasing burden of chronic diseases, such as neurodegenerative diseases and cancer. However, there is limited progress in finding effective treatment for these conditions. For this reason, members of the genus Streptomyces have been explored extensively over the past decades as these filamentous bacteria are highly efficient in pr...

Filamentous actinomycetes are commercially widely used as producers of natural products (in particular antibiotics) and of industrial enzymes. However, the mycelial lifestyle of actinomycetes, resulting in highly viscous broths and unfavorable pellet formation, has been a major bottleneck in their commercialization. Here we describe the successful morphological engineering of industrially impor...

redD encodes the transcriptional activator of the biosynthetic pathway for undecylprodigiosin, a red-pigmented, mycelium-bound antibiotic made by Streptomyces coelicolor A3(2) and Streptomyces lividans. A promoterless version of redD preceded by the efficiently used tuf1 ribosome binding site was inserted into two different plasmid vectors, providing a convenient reporter of transcriptional act...

While transcriptional regulation of the primary and secondary metabolism of the model organism Streptomyces coelicolor is well studied, little is still known about the role small noncoding RNAs (sRNAs) play in regulating gene expression in this organism. Here, we report the identification of a second target of the sRNA scr5239, an sRNA highly conserved in streptomycetes. The 159 nt long sRNA bi...

Plasmid pIJ7021) is a useful vector for the cloning and expression of streptomycete and mammalian genes in Streptomyces lividans.2~4) pIJ702 or its derivatives have broad host range, replicating in many Streptomyces species and in other actinomycetes (see ref 2~6 and refs therein). We have been interested in expanding the utility of pIJ702 by making it transducible. We cloned a segment of bacte...

The present study reveals that the effect of extra cellular protein isolated from Psychrophilic Actinomcetes against clinical pathogens. Totally 13 Actinomycetes were isolated from glacier soil. Among them the genus Streptomycetes and Micromonospora were isolated highly. MM9 medium was used as fermentation medium and the antimicrobial peptide activity was performed by disc diffusion method. Out...