Abstract The outbreak of new viral strains promotes advances in universal diagnostic techniques for detecting infectious diseases with unknown sequence. Long double-stranded RNA (dsRNA), a hallmark infections, serves as virus marker prompt detection viruses genomes. Here, we report on-chip paper electrophoresis ultrafast screening diseases. Negatively charged RNAs pass through the micro and nan...

Introduction The presence of double stranded RNA (dsRNA) in virus-infected tissue has been well documented by Morris and Dodds (4), Dodds and Bar-Joseph (2). The analysis of dsRNA proved to be useful for determining the virus-free status of propagation material after sanitation treatments. Isolated directly from grapevine, dsRNA is also a good source of viral RNA for synthesizing and cloning cD...

Insects and ectoparasites are causes for major concern throughout the world due to their economic welfare impacts on livestock agriculture. Current control measures involve chemicals such as acaricides which pose challenges like chemical resistance longer withholding periods. To enable more sustainable agriculture practices, it is important develop technologies that combine targeted effectivene...

Caenorhabditis elegans mutants deleted for TDP-1, an ortholog of the neurodegeneration-associated RNA-binding protein TDP-43, display only mild phenotypes. Nevertheless, transcriptome sequencing revealed that many RNAs were altered in accumulation and/or processing in the mutant. Analysis of these transcriptional abnormalities demonstrates that a primary function of TDP-1 is to limit formation ...

In most double-stranded RNA (dsRNA) viruses, RNA transcription occurs inside a polymerase (Pol) complex particle, which contains an RNA-dependent RNA Pol subunit as a minor component. Only plus- but not minus-sense copies of genomic segments are produced during this reaction. In the case of phi6, a dsRNA bacteriophage from the Cystoviridae family, isolated Pol synthesizes predominantly plus str...

The replication and transcription of double-stranded RNA (dsRNA) viruses occur within a polymerase complex particle in which the viral genome is enclosed throughout the entire life cycle of the virus. A single protein subunit in the polymerase complex is responsible for the template-dependent RNA polymerization activity. The isolated polymerase subunit of the dsRNA bacteriophage phi6 was previo...

Betanodavirus B2 belongs to a group of functionally related proteins from the sense-strand RNA virus family Nodaviridae that suppress cellular RNA interference. The B2 proteins of insect alphanodaviruses block RNA interference by binding to double-stranded RNA (dsRNA), thus preventing Dicer-mediated cleavage and the subsequent generation of short interfering RNAs. We show here that the fish bet...

The unprecedented target-specificity of double-stranded RNA (dsRNA), due to its sequence-specific mode action, puts dsRNA at the forefront biosafe insecticide technology. Since 2007, sensitive target genes have been identified in numerous crop pest insects, with an end goal applying interference (RNAi) management. Key RNAi targets include involved (1) feeding and digestion, (2) production dsRNa...

Cell-to-cell movement is essential for plant viruses to systemically infect host plants. Plant viruses encode movement proteins (MP) to facilitate such movement. Unlike the well-characterized MPs of DNA viruses and single-stranded RNA (ssRNA) viruses, knowledge of the functional mechanisms of MPs encoded by double-stranded RNA (dsRNA) viruses is very limited. In particular, many studied MPs of ...

Plants, fungi, and many other eukaryotes have evolved an RNA interference (RNAi) mechanism that is key for regulating gene expression the control of pathogens. RNAi inhibits expression, in a sequence-specific manner, by recognizing deploying cognate double-stranded (dsRNA) either from endogenous sources (e.g. pre-micro RNAs) or exogenous origin viruses, dsRNA, small interfering RNAs, siRNAs). R...