Upon tryptophan induction of tna operon expression in Escherichia coli, the leader peptidyl-tRNA, TnaC-tRNA(2)(Pro), resists cleavage, resulting in ribosome stalling at the tnaC stop codon. This stalled ribosome blocks Rho factor binding and action, preventing transcription termination in the tna operon's leader region. Plasmid-mediated overexpression of tnaC was previously shown to inhibit cel...

Icing and stalling on the surfaces of aircraft wings endanger flight safety. The object this research is a nanosecond pulsed dielectric-barrier-discharge plasma actuation unit with distributed layout, aerodynamic performance wing under icing conditions improved by changing electrical parameters. Actuated low voltage frequency, acts as sensor to judge according change in high discharge severe ge...

DNA cytosine methylation is an important epigenetic mechanism that is involved in transcriptional silencing of developmental genes. Several molecular pathways have been described that interfere with Pol II initiation, but at individual genes the molecular mechanism of repression remains uncertain. Here, we study the molecular mechanism of transcriptional regulation at Hox genes in dependence of...

Our genome contains many G-rich sequences, which have the propensity to fold into stable secondary DNA structures called G4 or G-quadruplex structures. These structures have been implicated in cellular processes such as gene regulation and telomere maintenance. However, G4 sequences are prone to mutations particularly upon replication stress or in the absence of specific helicases. To investiga...

Replication fork stalling generates a variety of responses, most of which cause an increase in single-stranded DNA. ssDNA is a primary signal of replication distress that activates cellular checkpoints. It is also a potential source of genome instability and a substrate for mutation and recombination. Therefore, managing ssDNA levels is crucial to chromosome integrity. Limited ssDNA accumulatio...

Abstract Remdesivir is the only FDA-approved drug for treatment of COVID-19 patients. The active form remdesivir acts as a nucleoside analog and inhibits RNA-dependent RNA polymerase (RdRp) coronaviruses including SARS-CoV-2. incorporated by RdRp into growing product allows addition three more nucleotides before synthesis stalls. Here we use synthetic chemistry, biochemistry cryo-electron micro...

Senescence represents a major tumour suppressor checkpoint activated by telomere dysfunction or cellular stress factors such as oncogene activation. In this issue of The EMBO Journal, Suram et al (2012) reveal a surprising interconnection between oncogene activation and telomere dysfunction induced senescence. The study supports an alternative model of tumour suppression, indicating that oncoge...