The role of microbial urease in calcite precipitation was studied utilizing a recombinant Escherichia coli HB101 containing a plasmid, pBU11, that encodes Bacillus pasteurii urease. The calcite precipitation by E. coli HB101 (pBU11) was significant although its precipitation level was not as high as that by B. pasteurii. Addition of low concentrations (5-100 microM) of nickel, the cofactor of u...

Most engineering infrastructures, including municipal solid waste containment facilities, are situated in unsaturated zone. The use of chemical additives for soil improvement has been linked to the adverse environmental issues being experienced globally. Research into more sustainable methods led discovery a novel and innovative method known as microbial induced calcite precipitation (MICP). La...

Sporosarcina globispora W 25(T) (DSM 4) is a Gram-positive, round-spore-forming, and psychrophilic bacterium. Here, we report the 5.66-Mb genome sequence of S. globispora W 25(T), which will accelerate the application of this psychrophile and provide useful information for genomic taxonomy and phylogenomics of Bacillus-like bacteria.

The morphological and physiological characteristics of 20 isolates of psychrophilic Bacillus were compared with 29 strains representing nine species of mesophilic Bacillus and 2 strains of Sporosarcina ureae to determine the taxonomic position of the psychrophiles. The psychrophiles formed four distinct groups which were sufficiently different from the mesophiles to warrant their designation as...

Numerous engineering and environmental issues can be resolved using the bacterial-induced calcite precipitation (BCP), which has potential to environmentally friendly, sustainable, economical. In BCP, bacterial enzymes used substrates divalent cations bind negatively charged ions surface produce biocementation. Various metabolic pathways involved in ureolysis are principal that have been illust...

We sequenced genomes of two ureolytic bacteria, Bacillus sp. JH7 and Sporosarcina sp. HYO08, which were isolated from concrete waste and have a potential for biocementation applications.

Beton yapıların hizmet ömrünü etkileyen en önemli faktörlerden biri erken yaşta oluşan çatlaklardır. Çatlak onarımı için kullanılan klasik yöntemlerin uygulanması zaman almakla beraber, hızlı müdahale olasılığı da düşüktür. Bu yöntemler büyük çatlakların uygundur, ancak çatlaklar inceldikçe bu onarım malzemelerinin, çatlağın derinlerine inmesi zorlaşmaktadır. Son yıllarda yapılan araştırmalar k...