نتایج جستجو برای: spindle

تعداد نتایج: 26260  

Journal: :Molecular biology of the cell 2005
Cristiana Mollinari Jean-Philippe Kleman Yasmina Saoudi Sandra A Jablonski Julien Perard Tim J Yen Robert L Margolis

The temporal and spatial regulation of cytokinesis requires an interaction between the anaphase mitotic spindle and the cell cortex. However, the relative roles of the spindle asters or the central spindle bundle are not clear in mammalian cells. The central spindle normally serves as a platform to localize key regulators of cell cleavage, including passenger proteins. Using time-lapse and immu...

Journal: :The Journal of Cell Biology 2000
Diana L. Walker Dong Wang Ye Jin Uttama Rath Yanming Wang Jørgen Johansen Kristen M. Johansen

A spindle matrix has been proposed to help organize and stabilize the microtubule spindle during mitosis, though molecular evidence corroborating its existence has been elusive. In Drosophila, we have cloned and characterized a novel nuclear protein, skeletor, that we propose is part of a macromolecular complex forming such a spindle matrix. Skeletor antibody staining shows that skeletor is ass...

2000
Sanne Jensen Marisa Segal Duncan J. Clarke Steven I. Reed

In Saccharomyces cerevisiae , the metaphase– anaphase transition is initiated by the anaphasepromoting complex–dependent degradation of Pds1, whereby Esp1 is activated to promote sister chromatid separation. Although this is a fundamental step in the cell cycle, little is known about the regulation of Esp1 and how loss of cohesion is coordinated with movement of the anaphase spindle. Here, we s...

Journal: :The Journal of Cell Biology 2001
Sanne Jensen Marisa Segal Duncan J. Clarke Steven I. Reed

In Saccharomyces cerevisiae, the metaphase-anaphase transition is initiated by the anaphase-promoting complex-dependent degradation of Pds1, whereby Esp1 is activated to promote sister chromatid separation. Although this is a fundamental step in the cell cycle, little is known about the regulation of Esp1 and how loss of cohesion is coordinated with movement of the anaphase spindle. Here, we sh...

Journal: :International review of cytology 2007
Kristen M Johansen Jørgen Johansen

The concept of a spindle matrix has long been proposed to account for incompletely understood features of microtubule spindle dynamics and force production during mitosis. In its simplest formulation, the spindle matrix is hypothesized to provide a stationary or elastic molecular matrix that can provide a substrate for motor molecules to interact with during microtubule sliding and which can st...

2015
Kian-Yong Lee Behrooz Esmaeili Ben Zealley Masanori Mishima

During animal cell division, the central spindle, an anti-parallel microtubule bundle structure formed between segregating chromosomes during anaphase, cooperates with astral microtubules to position the cleavage furrow. Because the central spindle is the only structure linking the two halves of the mitotic spindle, it is under mechanical tension from dynein-generated cortical pulling forces, w...

2003

The spindle of the colonial diatom Fragilaria contains two distinct sets of spindle microtubules (MTs): (a) MTs comprising the central spindle, which is composed of two half-spindles interdigitated to form a region of "overlap;" (b) MTs which radiate laterally from the poles. The central spindles from 28 cells are reconstructed by tracking each MT of the central spindle through consecutive seri...

2012
Shigeru Matsumura Mayumi Hamasaki Takuya Yamamoto Miki Ebisuya Mizuho Sato Eisuke Nishida Fumiko Toyoshima

Despite the growing evidence for the regulated spindle orientation in mammals, a systematic approach for identifying the responsible genes in mammalian cells has not been established. Here we perform a kinase-targeting RNAi screen in HeLa cells and identify ABL1 as a novel regulator of spindle orientation. Knockdown of ABL1 causes the cortical accumulation of Leu-Gly-Asn repeat-enriched-protein...

2016
Karen Perry McNally Michelle T. Panzica Taekyung Kim Daniel B. Cortes Francis J. McNally

In a wide range of eukaryotes, chromosome segregation occurs through anaphase A, in which chromosomes move toward stationary spindle poles, anaphase B, in which chromosomes move at the same velocity as outwardly moving spindle poles, or both. In contrast, Caenorhabditis elegans female meiotic spindles initially shorten in the pole-to-pole axis such that spindle poles contact the outer kinetocho...

Journal: :International Journal of Precision Engineering and Manufacturing 2022

This study investigates machining stability considering the rotational and thermal effects of a spindle-bearing system. Conventional method for analysis only has regarded static response spindle system, which does not represent actual conditions as some investigations mentioned. Bearing stiffness change caused by contact angle is applied to analytical approach dynamics At same time, in frequenc...

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