A total of 120 ejaculates from six adult Black Bengal bucks were studied to determine the semen attributes and evaluate their fertility. The various semen attributes of fresh semen were volume, sperm concentration, mass motility, live spermatozoa and normal spermatozoa percentage. All the semen attributes differed significantly (P<0.05) except normal spermatozoa percentage between the bucks. A ...

The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and r...

Very little is known about the effects of manipulating toad sperm activity in vitro, and such information is important in the development of a genetic resource bank for bufonid species. The specific objectives of this study were to: 1). identify the optimal inactivation and reactivation solutions for toad spermatozoa collected in urine; 2). establish the length of time toad spermatozoa can be e...

This study was performed to evaluate the effectiveness of three different conditions of osmotic pressure (325, 365 and 385 mOsm kg-1) in combination with dimethyl sulfoxide (DMSO) and NaCl or glucose on spermatozoa DNA damage, rate and duration of motility. Sperm was collected from eight healthy mature Mesopotamian catfish, evaluated microscopically and pooled at 25 °C. The pooled sperm samples...

Despite available information on the roles of osmotic pressure, potassium (K + ), and calcium (Ca 2+ ) in activation Eurasian burbot spermatozoon motility, changes milt biochemical composition mechanisms underlying their at temperatures above optimal spawning temperature is still unclear. We explored swelling, lipid proteome relation to osmolality swimming medium. The result revealed that incre...

objective(s):althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm dna fragmentation and infertility. the post-thaw survival of sperm...

Fundamental cryobiological characteristics of rhesus spermatozoa must be determined for successful cryopreservation techniques to be established. The main objectives of the present study were to determine the osmotic behavior and osmotic tolerance limits of rhesus macaque spermatozoa. Cell volume changes over anisotonic conditions were assessed using an electronic particle counter and sperm mot...

Aminopeptidase N (APN/CD13) and neutral endopeptidase (NEP/CD10) are enzymes present in human sperm cells and involved in regulation of sperm motility of noncapacitated spermatozoa. We investigated the involvement of APN/CD13 and NEP/CD10 in motility and in kinematic parameters of human capacitated spermatozoa. Sperm cells isolated by a discontinuous Percoll gradient (40%-80%) followed up by sw...

The aim of this work was to investigate the effect of live Staphylococcus aureus commonly present in the cervix of females on sperm motility and agglutination in vitro. Highly motile samples of spermatozoa (40 x 10 6 /ml) from normozoospermic males were co-incubated for 4h with 10 7 cells/ml of live S. aureus. After 30 minutes, 1, 2 and 4h of incubation, sperm motility and agglutination was che...