background: excess reactive oxygen species (ros) in the semen is believed to affect fertility in men. morphologically abnormal sperms and their relation to seminal oxidative stress in infertile and subfertile men are not clear. objective: to correlate various sperm morphological defects with seminal oxidative stress in infertile and subfertile men. materials and methods: the study included 25 p...

This study aimed at assessing the sperm quality of the Amazon catfish, Leiarius marmoratus ¸ after refrigeration without extenders. After capturing the animals and stripping of semen, the following parameters were analyzed: progressive motility, motility quality score, duration of motility and sperm morphology. An aliquot of fresh semen from each male was kept at room temperature (28 ± 2°C) as ...

Sperm selection techniques have became an important tool to improve sub fertile ejaculates. The main aim of this study was to assess the dynamics of motile sperm subpopulations of ejaculates from subfertile stallions subjected to density gradient centrifugation (DGC) involving a double layer colloid [DGC2], before and after storage for 24 and 48 hours. Ejaculates from eight stallions with ferti...

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

The semen analysis is the main diagnostic tool for evaluating the male fertility potential. The standard semen analysis includes evaluation of the sperm concentration, motility, and their morphology. The most important question is whether the results from semen analysis may be accurate markers for male fertility. Therefore, we retrospectively studied sperm quality among men attending the infert...

Background: This study was carried out to assess the effects of MnTBAP, a cell permeable antioxidant, on motility, membrane integrity, capacitation status and in vitro fertilization ability of frozen-thawed ram semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from five rams, mixed and divided into five equal fractions, and diluted (1:20 v/v) with commer...

Background: Artificial insemination is a valuable tool in genetic improvement programs and widely used breeding technique in farm animals. Semen processing and cryopreservation causes considerable damage to sperm DNA, motility apparatus, plasma membrane, acrosomal cap and thus, reduced fertility. Selenium is an integral part of glutathione peroxidase (GSH-Px), an enzyme which protects cell inte...

background: pentoxifylline (px) is a methyxanthin derivative that influences the sperm motion characteristics. in general, px has been reportedly effective in preserving sperm motility in vitro, also when administered orally to the asthenozoospermic patients. objective: the main objective of this prospective clinical trial study was to rule out the effect of oral administration of px on sperm p...