To evaluate the potential for fertilization by sperm injection into fish eggs, sperm from zebrafish, Danio rerio, were microinjected directly into egg cytoplasm of two different zebrafish lines. To evaluate physiological changes of gametes on the possible performance of intracytoplasmic sperm injection (ICSI), four different combinations of injection conditions were conducted using activated or...

Intracytoplasmic sperm injection (ICSI) is the latest of several microfertilization techniques that have been utilized predominantly to overcome severe male subfertility, giving fertilization and term pregnancy rates similar to conventional in-vitro fertilization (IVF) (but for other indications). Even though available data on children born after ICSI are very encouraging, the procedure must st...

For many years, the management of male-factor infertility has been empirical rather than evidence-based. In current clinical practice, assisted reproductive techniques are the most successful methods of alleviating male-factor infertility. To date, it remains unclear what adjuvant actions can be taken to improve the outcome of assisted reproductive techniques for male-factor infertility. Eviden...

Spermatid microinjection into oocytes was applied in cases of intracytoplasmic sperm injection (ICSI)/testicular sperm extraction (TESE) where no spermatozoa could be found in numerous testicular samples. Although several pregnancies were obtained with this procedure, serious concerns remain regarding its safety. Although the relevance of the injection of spermatids is by no means certain, we w...

Assisted reproductive techniques have been a great blessing for the infertile couple. Some have questioned their use, citing the unknown risk to the unborn child. The emergence of one of the newest techniques, intracytoplasmic sperm injection, has again sparked debate on the ethics of assisted reproductive techniques. Those who oppose intracytoplasmic sperm injections use are primarily concerne...

background: recent studies of uterine contractility in ivf�embryo transfer led us to consider an alternative, and possibly complementary, explanation for the high implantation rates of blastocysts. it has been demonstrated that myometrial contractile activity influences embryo implantation, possibly through mechanical displacement of embryos. objective: the aim of this study was to examine the ...

background: implantation remains a limiting step in ivf/icsi. endometrial injury isa promising procedure aiming at improving the implantation and pregnancy rates after ivf/icsi. objective: the aim of this study was to evaluate the effect of endometrial injury induced in precedingcycle on ivf/icsi outcome. materials and methods: four hundred patients undergoing their first ivf/icsi cycle in two ...