For artificial insemination (AI), boar semen is prepared in rather simple extenders, kept at 16-20°C and usually used in liquid form for up to 3 days from extension. Routine freezing of boar semen is occasionally used for gene banking, replacement of lost genetics or long-distance transport [1]. The reason behind this meagre use of freezing is the low tolerance of the ejaculated boar sperm to c...

Background Cryopreservation procedure for poultry semen is not reliable due to structural and biochemical damages which can lead to reduction in sperm quality and fertility. Development of cryopreservation medium has crucial effects for successful recovery of sperm after freezing. The objective of this study was assessment of cryopreservation of rooster semen using hand-made cryopreservation me...

The wood frog ( Rana sylvatica) is a freeze-tolerant species that encounters subzero temperatures during its winter breeding season, whereas the leopard frog ( R. pipiens) is freeze intolerant and breeds in spring. Osmotic and freezing tolerances of spermatozoa from these species were inferred from spermolysis rate, integrity of the plasma membrane as judged using vital dye assay, and motility ...

We evaluated the influence of two cooling rates (from 25 to 5 degrees C) on post-thaw function of frozen sperm in ocelots (Leopardus pardalis; n=3 males) and tigrinas (Leopardus tigrinus; n=4 males). Seven normospermic (>70% normal sperm) electroejaculates from each species were diluted with a 4% glycerol freezing medium, divided into two aliquots, and assigned to one of two cooling rates: fast...

Sperm motility stimulation is a pharmacological treatment used to improve sperm motility and treatment outcome in frozen-thawed epididymal and testicular spermatozoa. Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular adenosine triphosphate (ATP; molecule that ...

There has and will continue to be reproductive techniques available that have a positive impact upon the equine breeding industry. This review focuses on semen technologies that have been developed or are in the process of being developed. The use of fluorescent dyes and flow cytometry has provided the researcher and clinician with powerful tools to evaluate several sperm attributes. These proc...

A total of 30 ejaculates from three Hampshire crossbred boars were used to study the effect of freezing rates and thawing methods on quality of frozen boar semen. Semen was frozen using three freezing rates-30 0 c/min, 50 0 c/min and 70 0 c/min in a programmable biological semen freezer. The semen characteristics viz., sperm motility, live sperm, HOST reacted spermatozoa and intact acrosome wer...

Sperm motility stimulation is a pharmacological treatment used to improve sperm motility and treatment outcome in frozen-thawed epididymal and testicular spermatozoa. Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular adenosine triphosphate (ATP; molecule that ...

Sperm motility stimulation is a pharmacological treatment used to improve sperm motility and treatment outcome in frozen-thawed epididymal and testicular spermatozoa. Sperm motility is a critical factor in male fertility. Low motility can be caused by a variety factors including abnormal spermatogenesis, oxidative damage, or depletion of intracellular adenosine triphosphate (ATP; molecule that ...