introduction: during spermiogendsis, sperm chromatin histones are replaced by protamine. after fertilization, the sprem nucleus is decondenced by glutathione (gsh). in this survey, we study the effect of various concentrations of gsh on human in vitro sperm decondensation. material and methods: after preparation and analysis of semen samples, sperm pellet was washed by ham's f-10 medium twice a...

INTRODUCTION Although the pathophysiology of the testicular damage associated with varicocele remains unclear, sperm DNA damage has been identified as a potential explanation for this cause of male infertility. The current study was designed to determine the extent of sperm nuclear DNA damage in patients with varicocele, and to examine its relationship with parameters of seminal motility. MAT...

PURPOSE To date, standard semen parameters have been the only parameters investigated in sperm samples of infertile men in Algeria. We investigated, for the first time, semen parameters according to sperm DNA fragmentation (SDF) in these subjects. MATERIALS AND METHODS SDF was determined by a validated sperm chromatin dispersion test in 26 infertile men. Patients were split into two groups ac...

BACKGROUND Leptin and its receptor are present in spermatozoa; however, the role of leptin in sperm function is still controversial. Our present study aimed at demonstrating the effect of cryopreservation on sperm DNA fragmentation (DNAf) and investigating the possible effects of sperm capacitation techniques and leptin in vitro incubation on frozen-thawed sperm DNAf and oxidative stress. MET...

Introduction: The novel development of the (HBA) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. The purpose of this study was to evaluate the correlation between HBA score and histone deficiency, protamine content and DNA fragmentation. Methods: During April- March 2012 patients who were enrolled at ...

Changes in the morphology of the sperm nucleus in the egg cytoplasm are mong the immediate events in nucleocytoplasmic interactions during early embryogenesis. Soon after its entrance into the egg cytoplasm, the sperm nucleus of various organisms increases in size with the transformation of condensed chromatin to a diffuse state, resembling the chromatin of an interphase nucleus (2, 13, 15, 16)...

Background: During ICSI, motile sperm with normal morphology are selected and injected into oocyte. But integrity of chromatin is not observable. Therefore, researchers are trying for to present novel method of sperm selection with high percentage of normal chromatin. HOST test is a useful assay of functional integrity of the human sperm membrane. We decide to use HOST test after DGC for infert...

OBJECTIVE The present study was designed to determine the effect of the freeze-thawing procedure, computer controlled rate freezing and duration for six months, on human sperm chromatin (assessed by acridine orange test), vitality, motility, and morphology. DESIGN Experimental study MATERIAL AND METHOD Twenty semen samples were obtained from patients attending the infertility unit. The seme...