Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans. We investigated whether semen preparation using a combination of DGC and swim-up techniques could effectively decrease morphologically abnormal human sperms at the ultrastructural level. Semen samples were obtained from 16 infertile males and fract...

background: stress is a cause of male infertility. although sex hormones and sperm quality have been shown to be low in stress, sperm physiology and testicular functional proteins, such as phosphotyrosine proteins, have not been documented. objective: to investigate the acrosome status and alterations of testicular proteins involved in spermatogenesis and testosterone synthesis in chronic stres...

In this study, two following experiments were performed to improve post-thaw motility and viability of frozen-thawed ram spermatozoa. We examined i) the effects of different concentrations of bovine serum albumin (0, 0.3, 1, 5, 10 and 15% BSA) in semen diluents lacking egg yolk and ii) the effects of four semen diluents, fructose (F: control) and trehalose (T) in semen diluents containing egg y...

Background: Diabetes mellitus, a condition of chronic hyperglycemia, represents one of the greatest concerns to modern global health. Diabetes is a serious metabolic disorders with numerous complications. It is well known that, elevation of blood glucose levels leads to structural and functional changes in various target tissues and organs. The objective of the present study was to verify if th...

The study was designed to investigate the efficacy of treating the adult male rats with royal jelly (1g/kg B. Wt. orally) for one month with or without hydrogen peroxide (0.5%) in drinking water on sexual efficiency, glutathione and malondialdehyde tissue testis levels. The current study demonstrated that male rats receiving hydrogen peroxide caused a significant decrease (P<0.05) in the sperm ...

Supplementation of vitamin E (α-tocopherol) as an antioxidant into extender is useful to maintain sperm quality during the process cryopreservation by protecting sperms from reactive oxygen species (ROS). This study aims evaluate protective impacts supplementation on motility, viability, and reducing abnormality in Sapera goats. The sample semen used present was obtained fresh good-quality ejac...

The process of gametogenesis in the male occurs within the seminiferous tubules of the testes, resulting in the production of sperm. Many factors like Drugs, chemotherapy and toxins can generate harmful effect on spermatogenesis and affect sperm normal production. Gentamycin is a synthetic antibacterial agent belonging to the family of amino glycoside. Antioxidants scavengers of free radicals c...

AIM This study was designed to evaluate the prophylactic effect of Nigella sativa (NS) treatment on toxic effects induced by lead acetate (LA) on the reproductive hormones, spermiogram and gonadal histology of rats. MATERIALS AND METHODS A total of 20 Sprague-Dawley rats were divided into four groups of five rats each. Group 1 (negative control [NC]) was the NC and was given distilled water, ...

background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...

Intracytoplasmic sperm injection, while typically effective for overcoming low or absent fertilization in couples with a clear abnormality of semen parameters, is frequently utilized in combination with assisted reproductive technologies for other etiologies of infertility in the presence of normal semen parameters. This committee opinion provides a critical review of the literature, where avai...