OBJECTIVE Trichomonas vaginalis analyte-specific reagent is a highly sensitive assay for T vaginalis detection. We report how this diagnostic innovation influenced the sexually transmitted infection ordering practice patterns of 20 subacute-care clinicians. METHODS T vaginalis, Neisseria gonorrhoeae, and/or Chlamydia trachomatis screening data were audited on female swab submissions when only...

Bacitracin may be used for presumptive differentiation of group A from other beta-hemolytic streptococci. Whatever criteria are used for the test, a small number of erroneous results will be obtained. With the 1,161 strains studied in this laboratory, using a zone of 10 mm or greater as indicative of group A was better than using Maxted's original criterion of any zone of inhibition for group A...

A new reagent for the oxidative cleavage of DNA, (1,4,7-trimethyl-1, 4,7-triazacyclononane)iron(III) chloride was recently introduced. We have determined the utility of this reagent for detecting protein-DNA interactions within two types of complexes. Interestingly, we find that the rates of DNA cleavage by this reagent are differentially affected by the two classes of protein-DNA interactons s...

This paper outlines a more economical method in the serological identification of salmonella by using protein-A rich staphylococci sensitized with Salmonella polyvalent 0 and H antisera. Preliminary studies show that this method is simple, economical and as sensitive and specific as the original commercially prepared antisera with the exception of Salmonella typhi where there appears to be some...

A total of 147 preterm pregnant women at Orlando Regional Medical Center were screened for group B streptococci by using Lim Group B Strep Broth (GIBCO Laboratories, Madison, Wis.) and the Phadebact Strep B Test (Pharmacia Diagnostics, Piscataway, N.J.). Test results were available within 20 h of culture and, in the case of heavily colonized women, within 5 h. This procedure is useful in rapid ...