An Escherichia coli expression system has been developed for pea cytosolic ascorbate peroxidase (APX). The enzyme was expressed as a fusion product with the E. coli maltose-binding protein for rapid, affinity chromatography purification. Recombinant ascorbate peroxidase (rAPX) was purified by tryptic digestion to separate the maltose-binding protein from rAPX followed by three chromatographic s...

The effects of hyperthermia, coupling attributes and property variations on Low-density lipoprotein (LDL) transport within a multi-layered wall while accounting for the fluid structure interaction (FSI) is analyzed in this work. To understand the potential impact of the hyperthermia process, thermo-induced attributes are incorporated, accounting for the plasma flow, mass transfer, as well as th...

In order to study interdimer heme-heme electronic interactions in human hemoglobin, the Soret circular dichroism spectrum of the carboxy adduct is measured as a function of protein concentration, the spectrum at the highest concentration representing primarily that of alpha2beta2 tetramers (93%) and the lowest concentration representing primarily alphabeta dimers (68%). The tetramer-dimer diffe...

A sensing method based on resonance localized surface plasmon spectroscopy was developed for low molecular weight substrate and inhibitor molecules binding to heme proteins. Cytochrome P450 proteins have Soret and Q absorption bands in the visible wavelength region. The coupling between the molecular resonance of P450 and the localized surface plasmon resonance (LSPR) of functionalized silver n...

The Soret and Dufour effects on the steady, laminar mixed convection heat and mass transfer along a semi-infinite vertical plate embedded in a micropolar fluid saturated non-Darcy porous medium with heat and mass flux conditions are investigated. The non-linear governing equations and their associated boundary conditions are initially cast into dimensionless forms using similarity transformatio...

In this paper the thermal convective instabilities of a binary nanofluid is theoretically investigated. In order to analyze a binary nanofluid, an addition factor is proposed under linear stability theory which is applied to the stability analysis of normal fluid. The factor describes the effect of nanoparticles on the convective instability of a basefluid. The Soret effect which represents the...

An imidazolium tetrasubstituted cationic porphyrin derivative (the free base and its Zn(II) complex) with five-membered heterocyclic groups in the meso-positions were synthesized using microwave irradiation, and the compounds obtained characterized by (1)H-NMR and mass spectrometry. We observed that under microwave irradiation the yield is similar to when the synthesis is performed under conven...

Assimilatory NAD(P)H-nitrate reductase (EC 1.6.6.2) from Ankistrodesmus braunii has been purified to homogeneity by affinity chromatography on blue Sepharose. The specific activity of the purified enzyme is in the range of 72 to 80 units/mg of protein. The electronic spectrum of the native enzyme shows absorption maxima at 278, 414 (Soret), 532 (beta), 562 (alpha), and 669 nm and shoulders at 4...

Utilizing the Soret effect, we have employed a moderately focused laser beam (30 microm, 20 mW) to write spatial composition patterns into layers of the critical polymer blend poly(dimethyl siloxane)/poly(ethyl-methyl siloxane) (PDMS/PEMS, M(w)=16.4/22.8 kg/mol) both in the one- and in the two-phase region a few degrees above and below the critical temperature T(c)=37.7 degrees C. Because of th...

The effects of thermal radiation, Soret and Dufour parameters on mixed convection and nanofluid flow over a stretching sheet in the presence of a magnetic field are investigated. The flow is subject to temperature dependent viscosity and a chemical reaction parameter. It is assumed that the nanoparticle volume fraction at the wall may be actively controlled. The physical problem is modelled usi...