Background: there are no established methods for the preparation and standardization of Fusarium solani antigens. This lack of standardization makes it difficult to use these antigens in allergenic diagnostic tests. Objective: to obtain an appropriate standardized method for the preparation of the different antigen types of F. solani. M ethods: product ion of fungal extracts, follow ed by bioch...

Different assays to detect antigens of Trichinella spiralis during current infection have been standardized, although sensitivity values have been the main limitation to use them as routine diagnostic test. We report the production and use of monoclonal antibodies (MAbs) against the new born larvae which recognize both somatic and metabolic antigens from adult and muscular larvae (ML). We used ...

In a previous paper (Pijper et al., 1953) we described our investigations into the nature of the flagellum of Spirillum serpens. In Salmonella somatic, heat-stable or 0 antigens and flagellar, heat-labile or H antigens are recognized and give rise to typical agglutination patterns. H serum causes precipitates on bodies and flagella leading to fortuitous secondary entanglement of flagella, and 0...

Escherichia coli can be serotyped by determination of somatic (O), capsular (K), and flagellar (H) antigens, and clear associations exist between specific O antigens and pathogenic behavior. However, E. coli is very challenging to O type with traditional serologic methods, making new methods for E. coli somatic antigen detection highly desirable. Here, we describe a simple alternative molecular...

The term‘ paracolon ’ is passing out of common use and is applied here descriptively rather than definitively. Some strains were encountered which were members of the family Enterobacteriaceae and resembled coliform organisms in their morphological and cultural characteristics ; they were late, or non-lactosefermenters, and did not belong to Escherichia, Providence, Bethesda-Ballerup or other s...

In most of the bacterial agglutinating systems previously studied by the quantitative method (1-5) well defined capsular polysaccharide antigens and their corresponding antibodies were principally involved. The greater part of the antibody produced as a result of intravenous injections of microorganisms such as type-specific pneumococci or It. influenzae reacts with these capsular antigens, rel...

The present investigation examined the specificity of the salivary immune response of axenic and conventional mice to topically administered Salmonella typhi, S. gallinarum, and S. typhimurium. Specific antibacterial antibodies were determined by passive hemagglutination and bacterial agglutination. Reciprocal antibody titers up to 320 were detected in saliva from mice immunized and assayed wit...

The character of the antibody response in the rabbit to Salmonella typhimurium somatic (O) antigen was similar to the response to each of several serotypes of Shigella flexneri O antigens, namely a predominance of production of immunoglobulin M (IgM) antibody. Lipopolysaccharide protein (LPSP) and lipopolysaccharide (LPS) fractions of Salmonella O antigen differed significantly in both quantita...