zagros (drought tolerant) and pishtaz (drought susceptible) cultivars were classified on the basis of shoot dry weight and were used as plant material in this study. total chlorophyll, carotenoids, antocyanins, proline, soluble sugar contents, lipid peroxidation, antioxidant enzyme activities and protein patterns were determined. seedlings of wheat genotypes were grown in nutrient solution cult...

It is generally assumed that the primary role of phloem loading is to drive long-distance transport by elevating hydrostatic pressure in sieve elements. This concept is consistent with the fact that, in many plants, energy is used to increase the concentrations of photoassimilates in the leaf phloem to levels well above those in mesophyll cells. However, on the basis of recent data, these funda...

In this study the enzymatic activity of Mycoplasma agalactiae MAG_5040, a magnesium-dependent nuclease homologue to the staphylococcal SNase was characterized and its antigenicity during natural infections was established. A UGA corrected version of MAG_5040, lacking the region encoding the signal peptide, was expressed in Escherichia coli as a GST fusion protein. Recombinant GST-MAG_5040 exhib...

In this work, four vacuolar H+-PPase (VHP) genes were identified in the grape genome. Among them, VvVHP1; 2 was strongly expressed in berry skin and its expression exhibited high correlations to anthocyanin content of berry skin during berry ripening and under ABA and UVB treatments. VvVHP1; 2 was transcriptionally activated directly by VvMYBA1, and VvVHP1; 2 overexpression promoted anthocyanin...

Hunger is a powerful drive that stimulates food intake. Yet, the mechanism that determines how the energy deficits that result in hunger are represented in the brain and promote feeding is not well understood. We previously described SLC5A11-a sodium/solute co-transporter-like-(or cupcake) in Drosophila melanogaster, which is required for the fly to select a nutritive sugar over a sweeter nonnu...

A carbohydrate-transport assay was developed that does not require isotopically labelled substrates, but allows transport reactions to be followed spectrophotometrically. It makes use of a membrane system (hybrid membranes or proteoliposomes) bearing the transport system of interest, and a pyrroloquinoline quinone-dependent aldose dehydrogenase [soluble glucose dehydrogenase (sGDH)] and the ele...

Mycobacterium tuberculosis (Mtb), the aetiological agent of tuberculosis, has evolved to scavenge nutrients from the confined environment of host macrophages with mycobacterial ATP-binding cassette (ABC) transporters playing a key role in nutrient acquisition. Mtb-UspC (Rv2318) is the solute-binding protein of the essential transporter UspABC, one of four Mtb ABC transporters implicated by homo...

serosal permeability to sugars (Holman & Naftalin, 1975). With a preparation of the mucosa of rabbit ileum and the sugar galactose, these authors were able to show that, although theophylline and dibutyryl cyclic AMP had no effect on the entry or exit permeabilities to galactose at the mucosal pole of the epithelium, both the serosal entry and the exit permeability were decreased to 30% of cont...