Polarizable Continuum Model (PCM) analysis has been carried out using B3LYP method with 6-311++G(d,p) basis set for three water soluble vitamins (biotin, L-ascorbic acid and pyridoxine) and one fat soluble vitamin (phylloquinone) in ten solvents with wide range of dielectric constants. In this paper, we report electrostatic, dispersion and repulsive interaction components of Gibb’s free energy ...

The high-performance liquid chromatography (HPLC) method with photodiode array (PDA) and fluorescence (FL) detection for the simultaneous separation and determination of caffeine and water-soluble vitamins is described. The method is relatively rapid: 13 compounds can be analyzed within 30 min: caffeine; ascorbic acid (vitamin C); thiamine (vitamin B1); riboflavin 5-phosphate (FMN, flavin monon...

Vitamins are important micronutrients that are often precursors to enzymes, which all living cells require to perform biochemical reactions. However, humans cannot produce many vitamins, so they have to be externally obtained. Using vitamin‐producing microorganisms could be an organic and marketable solution to using pseudo‐vitamins that are chemically produced, and could allow for the producti...

This Application Note shows how to carry out qualitative and quantitative analysis of fat soluble vitamins from two different food matrixes. A robust reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous determination of nine fat soluble vitamins was developed. Separation and quantification was achieved by an Agilent 1260 Infinity LC System using an Agilent Por...