Soil bacterium DNA was isolated by minor modifications of previously described methods. After purification on hydroxyapatite and precipitation with cetylpyridinium bromide, the DNA was sheared in a French press to give fragments with an average molecular mass of 420,000 daltons. After repeated hydroxyapatite purification and precipitation with cetylpyridinium bromide, high-pressure liquid chrom...

28-hydroxymilbemycins, 1 3/?,30-dihydroxymilbemycins, and others.4~8) On the other hand, Ramos Tomboet al. have reported 13/Miydroxylation and 14, 1 5-epoxidation of milbemycins by Streptomyces violascens ATCC31560.9) During our screening of microbial conversion of milbemycin A4 (la), we observed that an actinomycete, strain SANK65087, newly isolated from soil, converted milbemycin A4 (la) to a...

Muramic acid, glucosamine, mannosamine and galactosamine in soils may be useful for elucidating the microbial origin of soil organic nitrogen. Therefore, a method was developed to determine the aldononitrile acetate derivatives of the four amino sugars simultaneously in the 6 M HCl hydrolysates of soil samples by means of high resolution gas li uid chromatography. This method was sensitive enou...

Forensic crime scene sample analysis, by its nature, often deals with samples in which there are low amounts of nucleic acids, on substrates that often lead to inhibition of subsequent enzymatic reactions such as PCR amplification for STR profiling. Common substrates include denim from blue jeans, which yields indigo dye as a PCR inhibitor, and soil, which yields humic substances as inhibitors....

A multiresidue method for simultaneous determination of four pesticides (diazinon, acetochlor, aldrine and carbofuran) belonging to different pesticide groups, extracted from soil samples, is described. The method presented is based on liquid-solid extraction (LSE) and determination of pesticides, i.e. the pesticides were extracted by methanol-acetone mixture, purified on florisil column and el...

Francisella tularensis is the etiologic agent of the zoonotic disease tularemia and is thought to be maintained in the environment principally by various terrestrial and aquatic vertebrate animals. The organism is known to persist in water or mud for long periods of time and Francisella-specific DNA has been identified from water and soil. To gain a better understanding of the ecology and epide...

Using the principles and methods of eco-economics as research object, Aral City comprehensively expounds ecological service functions such ecosystem regulation climate, carbon sequestration, soil conservation, water conservation purification environment, evaluates its economic value.The total value estimated 2021 is 1303.65 million yuan. At same time, importance urban ecosystems, from large to ...