نتایج جستجو برای: six sets yielded polymorphic and reproducible bands
تعداد نتایج: 16867412 فیلتر نتایج به سال:
Knowledge of genes action on important traits and their breeding value is necessary to achieve high yielding cultivars in food crops. Molecular markers has eliminated the need for knowing the pedigree of genotypes for estimating Kiniship matrix required to estimate breeding values of taits of interest. In this research, 97 genotypes of maize were evaluated for 17 different agronomic triats usin...
Genetic diversity of two wild Kalibaus, Labeo calbasu populations and one hatchery stock was studied using random amplified polymorphic DNA (RAPD) method. The three 10–mer random primers (OPA01, OPB02 and OPC03) yielded a total of 26 reproducible and consistently scorable RAPD bands of which 15 (57.69%) were considered as polymorphic (P95) indicating a high level of genetic variation in all the...
Three Iranian native strains and three Japanese commercial lines of the silkworm Bombyx mori were analyzed using amplified fragment length polymorphism (AFLP) markers. A set of ten PstI/TaqI primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. Estimates of Nei’s gene diversity for all loci in individual strains and commercial lines indicated a higher degre...
genetic diversity is the basis success of improving the quality and quantity of crops. recognizing of genetic diversity and genetic potential of each plant species in plant breeding is necessary. in this study genetic diversity of 30 lentil samples belonging to collection of the college of agriculture of krage were evaluated using rapd molecular markers. rapd products were scored for presence (...
In order to detect genetic variation of native fowls in Mazandaran native Fowls breeding station, blood samples were collected from 100 male and female of birds (1:11). The DNA of the blood samples was extracted according to an optimized salting out protocol. The extracted DNA was amplified through polymerase chain reaction (PCR). Of the twenty random primers (10 mer) were used in this study, f...
dna methylation as epigenetic mark plays a key role in normal differential and developmental processes as well as in dynamic gene regulation at the genomic level. to assess dna methylation pattern in different developmental stages of aeluropus littoralis, methylation sensitive amplified polymorphism (msap) was used. methylation and demethylation status at the ccgg recognition site were tracked ...
Assessment of genetic diversity is important step for exploitation from plant material in breeding programs. In this study, genetic diversity of 55 barley lines evaluated using SSR markers and also microsatellite markers were used to identify informative markers associated with phonological and yield traits related to drought escape including days to heading, grain filling period, days to physi...
The molecular marker (SSR) has been used to investigate the markers associated with the agronomic traits including days to 50% flowering, tube ring time, days to maturity, plant height, the number of main stems per plant, the number of tubers per plant, dry matter content, main stem diameter, a single tuber weight, average single tuber weight, and the total yield in potato genotypes. Ten primer...
ISSR markers were used to determine the genetic variation and sex determination of the three life-stages of 41 samples of Gracilaria corticata from two regions of the Persian Gulf and Oman Sea namely Bostaneh and Lipar. The specimens were cultured in PES media to observe the different life stages. DNA was extracted by modified CTAB method. After screening of 20 ISSR primers, four primers were s...
Genetic Diversity and Phylogenetic Relationship among three Duck Breeds and Geese using RAPD Markers
| Poultry and poultry products are the most economical protein sources for the ever-increasing human population and to meet the future food security. While these are promising food sources, the importance of genetically improved avian species in the breeding programme has increased significantly. In this study, we applied randomly amplified polymorphic DNA (RAPD) methodology to elucidate geneti...
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