The starting point for this paper is a comparison undertaken by Romm and Wong (1997) between two data sets focusing on the strategy of organizational web site design. The first data set, comprising two case studies, originally documented by Jarvenpaa and Ives (1996), was based on the computer industry in the US. The second data set, comprising one case study, was based on a university in Austra...

We discuss the design and evaluation of a class of agents that we call adaptive web site agents. The goal of such an agent is to help a user find information at a particular web site, adapting its behavior in response to the actions of the individual user and the actions of other visitors to the web site. The agent recommends related documents to visitors. It integrates and coordinates among di...

Hydrophobins are surface-active fungal proteins that adsorb to the water-air interface and self-assemble into amphiphilic, water-repelling films that have a surface elasticity that is an order of magnitude higher than other molecular films. Here we use surface-specific sum-frequency generation spectroscopy (VSFG) and site-directed mutagenesis to study the properties of class I hydrophobin (HFBI...

The importance of Web site usability is apparent. Previous studies have found that a usable Web site creates a positive attitude toward online stores, increases stickiness and revisit rates, and eventually stimulates online purchases. A usable Web site also provides benefits to e-business by reducing website development, support, and maintenance costs. The ISO 9241-11 standard for usability has...

This paper presents techniques for identifying domain specific web sites that have been implemented as part of the EC-funded R&D project, CROSSMARC. The project aims to develop technology for extracting interesting information from domain-specific web pages. It is therefore important for CROSSMARC to identify web sites in which interesting domain specific pages reside (focused web crawling). Th...

Reports Purfication of Antibody to Galactosyl-Protein Conjugates: E. W. Bassett, S. M. Beiser, S. W. Tanenbaum ...... .................................. 1475 Age Changes in Adenine Nucleotides in Flight Muscle of Male House Fly: M. Rockstein and D. E. Gutfreund ....... .............................. 1476 Ether-Induced Retrograde Amnesia for One-Trial Conditioning in Mice: J. P. Abt, W. B. Essma...

OBJECTIVES To assess patients' usage of the Internet as a source of personal healthcare information and patients' perceptions of usefulness and content preferences of more locally focused online health-related material. MATERIALS AND METHODS A paper-based survey was undertaken by a convenience sample of cardiac outpatients. Age, gender, Internet access, Internet usage, perception of usefulnes...

We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flanking primers and one mutagenic primer with different melting temperatures (T(m)) are used togethe...

The Internet can be an effective alternative for data collection for multicenter studies. It has major advantages over the "classical" method of using paper and traditional ("snail") mail. We developed an Internet site and implemented collection of data for a multicenter study of ethical decision-making. The Web site was built with Microsoft FrontPage as the authoring tool. Database management ...

BACKGROUND Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate purification of DNA between two rounds of PCR. RESULTS The strategy relies on the use of a limiting concentration of one of the flanking primers (reve...