dna polymorphism within diacylglycerol transferase 2 (dgat2) / monoacyl glycerol transferases 2 (mogat2), leptin and butyrophilin genes were analysed using pcr-sscp in murrah buffalo. the single strand conformation polymorphism (sscp) analysis of amplified gene fragment in exon 5 of mogat2, exon 3 of leptin and intron 1 of butyrophilin gene revealed different patterns. a, b and c showed the fol...

Osmium tetroxide, 2,2'-bipyridine (Os,bipy) has been widely applied as a probe of the DNA structure. To obtain information about reactivity of DNA bases toward this probe synthetic homopolynucleotides poly(dT), poly(dC), poly(dG) and poly(dA) were treated with Os,bipy and the content of modified bases measured by stripping voltammetry and absorption spectrophotometry. After 20 hours' treatment ...

Background: Obesity is a very common disorder resulting from an imbalance between food intake and energy expenditure, and it has a substantial impact on the development of chronic diseases.  The aim of this study was to examine the association of INSIG2 (rs7566605) gene polymorphism with obesity and obesity associated phenotypes in North Indian subjects. Methods: The variants were investig...

The poly(A) site of bovine prolactin (bPRL) mRNA was examined by phased priming of cDNA synthesis with oligodeoxynucleotides of the general sequence d(pT8-N-N'). The existence of multiple poly(A)-adjacent sequences in bPRL mRNA was indicated by the production of specific chain-termination fragments with at least three d(pT8-N-N') sequences. Comparison of the sequence bands produced by initiatio...

RNA from a rat liver tumor (Morris hepatoma 5123tc) was used to construct cDNAs together comprising the complete coding sequence of rat oncomodulin mRNA. Information obtained from these cDNAs as well as from primer extension analysis gave a deduced length for the complete oncomodulin mRNA of approximately 680 nucleotides (excluding the poly(A) tail) including a 5'-untranslated region of 97 +/- ...

We report the labelling of dideoxy nucleotides (ddNTPs) for use in electrochemical array based primer extension for the detection of single nucleotide polymorphisms (SNPs). The results confirm the extension of the immobilised primers for each of the four ddNTPs, representing a significant advance in achieving a cost-effective platform for screening of disease-specific SNPs.

There is a debate concerning mono- or poly-phyletic origins of the Near Eastern crops. In parallel, some authors claim that domestication was not possible within the natural range of the wild progenitors due to wild alleles flow into the nascent crops. Here we address both, the mono- or poly-phyletic origins and the domestications within or without the natural range of the progenitor, debates i...

A cDNA clone for muscle adenylate kinase was isolated from a cDNA library of chick skeletal muscle poly(A)+ RNA, and the DNA sequence was determined. The cDNA insert had 854 nucleotides, which consisted of the 5'-untranslated sequence of 57 nucleotides, the sequence of 582 nucleotides coding for 194 amino acids, and the 3'-untranslated sequence of 163 nucleotides and the poly(A) tail of 52 nucl...

Poly-linker primer system for detection of single nucleotide polymorphisms and its application in genotyping hepatitis B virus Yongzhong Wang1,*,†, Bo-Yu Xue2,†, Longgen Liu2, Zhen Zhu1, Xin Xu1, Hongyu Zhang1, Xiangke Pu1, and Xiaoqin Liu1 Laboratory Centre, The Third People’s Hospital of Changzhou, Changzhou 213001, China, and University of Nanjing Traditional Chinese Medicine, Nanjing 210023...

Turkey Meat as Source of CC9/ CC398 Methicillin-Resistant Staphylococcus aureus in Humans? TO THE EDITOR—Livestock-associated methicillin-resistant Staphylococcus aure-us (MRSA) of clonal complex (CC) 398 were first reported to cause severe infections in humans in 2005 [1]. Direct animal exposure is considered the most effective means of MRSA CC398 transmission from livestock to humans. However...