A cDNA clone for muscle adenylate kinase was isolated from a cDNA library of chick skeletal muscle poly(A)+ RNA, and the DNA sequence was determined. The cDNA insert had 854 nucleotides, which consisted of the 5'-untranslated sequence of 57 nucleotides, the sequence of 582 nucleotides coding for 194 amino acids, and the 3'-untranslated sequence of 163 nucleotides and the poly(A) tail of 52 nucl...

Poly-linker primer system for detection of single nucleotide polymorphisms and its application in genotyping hepatitis B virus Yongzhong Wang1,*,†, Bo-Yu Xue2,†, Longgen Liu2, Zhen Zhu1, Xin Xu1, Hongyu Zhang1, Xiangke Pu1, and Xiaoqin Liu1 Laboratory Centre, The Third People’s Hospital of Changzhou, Changzhou 213001, China, and University of Nanjing Traditional Chinese Medicine, Nanjing 210023...

It has been shown earlier that the DNA double helix is opened due to a prolonged contact of the DNA molecule with the surface of the mercury electrode. At neutral pH, the opening process is relatively slow (around 100 s), and it is limited to potentials close to -1.2 V (against SCE). The opening of the double helix has been explained by strains in the DNA molecule due to strong repulsion of the...

Submit Manuscript | http://medcraveonline.com Abbreviations: PCa: Prostate Carcinoma; CRPC: Castrationresistant Prostate Carcinoma; MMR: Mismatch Repair; ADT: Androgen-deprivation Therapy; OS: Overall Survival; NER: Nucleotide Excision Repair; BER: Base Excision Repair; HDR: Homology Directed Repair; HR: Homologous Recombination; DDR: DNA Damage Response; NHEJ: Non-homologous End Joining; SSB: ...

this study was conducted in order to investigate the association between the single nucleotide polymorphism (snp) rs2305957 g/a and recurrent pregnancy loss (rpl) in a group of palestinian women residing in gaza strip. a retrospective case-control study was carried out during the period of may to august 2015. a total of 380 females including 190 recurrent pregnancy loss (rpl) patients and 190 c...

objective: although the etiology of schizophrenia is unknown, it has a significant genetic component. ‎a number of studies have indicated that neuregulin-1 (nrg1) gene may play a role in the ‎pathogenesis of schizophrenia. in this study, we examined whether the rs2439272 of nrg1 ‎is associated with schizophrenia and its negative symptoms in an iranian population.‎ method: rs2439272 was genotype...

purpose: neuromyelitis opitca (nmo) is an autoimmune disease that relates to deposition of anti-aquaporin-4 (aqp4) igg in the central nervous system (cns). however, called seronegative nmo when patients are negative for aqp4 antibody. nevertheless, nmo is most probably an antibody mediated disease. the aim of this study was to assess the association between seronegative nmo disease and variatio...

the rs2476601 (r620w, c1858t) polymorphism in ptpn22 gene has been repeatedly reported to be associated with rheumatoid arthritis (ra). the rs 2476601 is widely suggested for predictive testing and risk assessment for ra. the aim of this study was to test the possible association of this snp with ra in iranian population.a total of 872 samples (405 confirmed ra patients and 467 healthy controls...

mannose-binding lectin (mbl) is a ca⁺² -dependent collagenous lectin, that is produced by liver and mediates innate immune responses by opsonization of pathogens. the serum level of mbl varies widely among healthy individuals, ranging from 0.05 µg/ml (or lower) to over 5 µg/ml, mainly depending on genetic variation. this study has examined promoter and exon 1 of mbl2 genotype among 117 iranian ...

A novel primer designed to improve the specificity of PCR amplification, called the annealing control primer (ACP), comprises a tripartite structure with a polydeoxyinosine [poly(dI)] linker between the 3' end target core sequence and the 5' end nontarget universal sequence. We show that this ACP linker prevents annealing of the 5' end nontarget sequence to the template and facilitates primer h...