The present study describes approaches for the identification of individual beech trees using maternal tissues from their seeds or fruits. Four microsatellite markers were used for genetic analysis of seedlots from Fagus orientalis Lipsky, a highly out-crossing tree species. Seeds from 11 single-tree harvests belonging to one population, (7 seeds from each), as well as non-paranchymatic materna...

PURPOSE To identify the disease locus for autosomal recessive congenital cataract in a consanguineous Pakistani family. METHODS All affected individuals underwent detailed ophthalmologic and medical examination. Blood samples were collected and DNA was extracted. A genome-wide scan was performed with polymorphic microsatellite markers on genomic DNA from affected and unaffected family members...

PREMISE OF THE STUDY Polymorphic microRNA (miRNA)-based microsatellite markers were developed to investigate the genetic diversity and population structure of Nelumbo nucifera (Nelumbonaceae). METHODS AND RESULTS A total of 485 miRNA-based microsatellites were found from the genomic DNA sequences of N. nucifera. After several rounds of screening, 21 primer pairs flanking di-, tri-, or pentanu...

Microsatellite instability (MSI) has been found in a range of human tumors, and little is known of the links between MSI and herpesvirus. In order to investigate the relationship between MSI and Gallid herpesvirus 2 (GaHV-2)-induced lymphoma, fifteen Marek's disease (MD) lymphomas were analyzed through using 46 microsatellite markers, which were amplified by PCR from DNA specimens of lymphoma a...

Vol. 36, No. 3 (2004) BioTechniques 409 The process of microsatellite development and profiling involves three primary steps: (i) isolating regions of genomic DNA that contain microsatellite loci; (ii) developing strategies for screening each locus, which requires designing primers for amplification, optimizing reaction conditions, and screening for variation; and (iii) genotyping sampled indiv...

Sipunculus nudus, the peanut worm, is the best known species in its genus. This unsegmented subtidal marine worm is consumed in some parts of Asia and is also used as fish bait. We found 20 microsatellite DNA markers for S. nudus. The number of alleles per polymorphic locus ranged from two to seven in a sample of 39 individuals. Observed and expected heterozygosities per polymorphic locus...

PREMISE OF THE STUDY Polymorphic microsatellite markers were developed for the lichen species Cetraria aculeata (Parmeliaceae) to study fine-scale population diversity and phylogeographic structure. METHODS AND RESULTS Using Illumina HiSeq and MiSeq, 15 fungus-specific microsatellite markers were developed and tested on 81 specimens from four populations from Spain. The number of alleles rang...

Four Saudi Arabian indigenous sheep populations including Najdi, Hbsi, Arb, and Naemi were genotyped for 16 microsatellite markers recommended by the food and agriculture organization (FAO). This study aims to provide information on the genetic structure of the breeds analyzed and give indications and proposals for the cross breeding program. All loci were polymorphic in all populations and loc...

Fourteen microsatellite DNA markers were developed for studies of gene flow in the Neotropical rain forest tree Virola surinamensis. The loci were unlinked and polymorphic in a sample of 21 individuals, with two to 10 alleles per locus and observed heterozygosity ranging from 0.14 to 0.76. The overall exclusion probability (0.997) indicates high resolution for parentage-based analyses of gene f...